Microwave-assisted acid pretreatment for enhancing enzymatic saccharification of sugarcane trash
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ORIGINAL ARTICLE
Microwave-assisted acid pretreatment for enhancing enzymatic saccharification of sugarcane trash Euis Hermiati 1 & Raden Permana Budi Laksana 1 & Widya Fatriasari 1 & Lutfi Nia Kholida 2 & Ahmad Thontowi 2 & Yopi 2 & Diemas Rialdi Arnieyanto 3 & Verawat Champreda 4 & Takashi Watanabe 5 Received: 6 April 2020 / Revised: 24 July 2020 / Accepted: 19 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Sugarcane trash (ST) is an abundant but underused biomass left in the field when the sugarcane is harvested. However, there are only a few studies to evaluate pretreatment of the residual bioresources for enzymatic saccharification. In this study, we aimed to explore the effects of combined severity factor (CSF), resulted from the use of different acids (phosphoric, maleic, and oxalic acids) on the pretreatment efficiency in microwave (MW)-accelerated process. The effects of acids and CSF on pretreated biomass properties and sugars obtained in the pretreatment soluble fraction and in enzymatic hydrolysate were evaluated. The degree of crystallinity of biomass pretreated at the same CSF is different due to different of acids used, and it did not correlate with the sugars obtained in the hydrolysate after enzymatic saccharification. MW-assisted acid pretreatment of ST at CSF of 2.05 affected functional groups and morphology of the biomass significantly. ST exposed to the same severity (CSF 2.05) using different acids resulted in reducing sugars yield 25.3–30.8% (based on initial biomass) from the pretreatment soluble fraction and 26.1–28.8% (based on initial biomass) from enzymatic hydrolysis, with the highest (30.8% and 28.8%) when used maleic acid. We succeeded in the production of total reducing sugars by 59.6% yield (of initial biomass) from recalcitrant sugarcane trash by applying MW-assisted maleic acid pretreatment at CSF of 2.05. The work provided insights into the use of organic acid as the catalyst in MW-accelerated process and can be further developed for application in pretreatment of ST in industry. Keywords Combined severity factor . Microwave heating . Organic acid . Pretreatment . Sugarcane trash . Sugar yield
1 Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13399-020-00971-z) contains supplementary material, which is available to authorized users. * Euis Hermiati [email protected] 1
Research Center for Biomaterials, Indonesian Institute of Sciences, Cibinong Science Center, Jl. Raya Bogor Km 46, Cibinong, Bogor 16911, Indonesia
2
Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong Science Center, Jl. Raya Bogor Km 46, Cibinong, Bogor 16911, Indonesia
3
Department of Chemisty, Pakuan University, Jl. Pakuan Utara, Bogor 16143, Indonesia
4
National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, Pathum Thani 12120, Thailand
5
Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho
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