Assessment of proliferation, migration and differentiation potentials of bone marrow mesenchymal stem cells labeling wit
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ORIGINAL ARTICLE
Assessment of proliferation, migration and differentiation potentials of bone marrow mesenchymal stem cells labeling with silica-coated and amine-modified superparamagnetic iron oxide nanoparticles Dong Yao
. Na-na Liu . Bi-wen Mo
Received: 27 March 2019 / Accepted: 4 May 2020 Ó Springer Nature B.V. 2020
Abstract Superparamagnetic iron oxide nanoparticles have been widely used for cell labeling in preclinical and clinical studies, to improve labeling efficiency, particle conjugation and surface modifications are developed, but some modified SPIONs exert side-effect on physiological activity of cells, which cannot be served as ideal cell tracker. In this study, amine-modified silica-coated SPIO (SPIO@SiO2NH2, SPIO@S-N) nanoparticles were used to label bone marrow derived mesenchymal stem cells (BMD. Yao (&) The Laboratory of Respiratory Disease, The Affiliated Hospital of Guilin Medical University, Guilin 541000, China e-mail: [email protected] D. Yao B. Mo (&) Guangxi Colleges and Universities Key Laboratory of Respiratory Disease, Guilin 541000, China e-mail: [email protected] D. Yao Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Guilin Medical University, Guilin 541000, China
MSCs), then the stem cell potentials were evaluated. It was found BM-MSCs could be efficiently labeled by SPIO@S-N nanoparticles. After labeling, the BMMSCs viability kept well and the migration ability increased, but the osteogenesis and adipogenesis potentials were not impaired. In steroid associated osteonecrosis (SAON) bone defect model, stem cell implantation was performed by injection of SPIO@SN labeled BM-MSCs into marrow cavity locally, it was found the SPIO positive cells homed to the periphery of defect region in control group, but were recruited to the defect region in poly lactic-coglycolic acid/tricalcium phosphate (PLGA/TCP) scaffold implantation group. In conclusion, SPIO@S-N nanoparticles promoted migration while retained proliferation and differentiation ability of BM-MSCs, implying this kind of nanoparticles could be served not only an ideal tracking marker but also an accelerator for stem cell homing during tissue repair. Keywords Superparamagnetic iron oxide nanoparticles Mesenchymal stem cells Viability Migration Differentiation Steroid associated osteonecrosis
N. Liu Department of Basic Medical Sciences, School of Nursing, Sanmenxia Polytechnic, Sanmenxia 472000, China B. Mo The Second Affiliated Hospital of Guilin Medical University, Guilin 541100, China
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Cytotechnology
Introduction Mesenchymal stem cells (MSCs) derived from bone marrow are multipotent stromal cells that can differentiate into a variety of cell types, including adipocytes, osteocytes, chondrocytes and myocytes (Matthay et al. 2017; Pittenger et al. 1999; Zhao et al. 2011). These cells play a vital role in the repair and regeneration of various tissues and organs throughout the human body (Xi et al. 2013). Many studies show that
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