Effects of icariin on the proliferation and osteogenic differentiation of human amniotic mesenchymal stem cells
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(2020) 15:578
RESEARCH ARTICLE
Open Access
Effects of icariin on the proliferation and osteogenic differentiation of human amniotic mesenchymal stem cells Fang Wang1†, Zhiyong Yang2†, Wei He1, Qinggao Song1*, Kun Wang1 and Yali Zhou1
Abstract Background: Tissue engineering technology has been applied extensively for clinical research and human amnion mesenchymal stem cells (hAMSCs) could cause mesenchymal stem cells to differentiate into the bone tissue. However, it is necessary to develop and identify the safer appropriate amount of osteogenic inducer. The objective of this study is to investigate the effect of icariin (ICA) on the proliferation and osteogenic differentiation of hAMSCs. Methods: The morphology and phenotype of hAMSCs were discovered by flow cytometry and immunocytochemical staining. The osteogenic differentiation of hAMSCs under the influence of different concentrations of ICA were assessed by alkaline phosphatase (ALP) activity substrate assay and alizarin red staining. Results: MTT assay revealed that the hAMSCs pretreated with ICA exhibited increased proliferation when compared with the control group, and the most optimum concentration of ICA was 1 × 10− 6 mol/L. The combined analysis of ALP activity and ARS staining showed that ICA could significantly promote the osteogenic differentiation of hAMSCs, and the effect was most significant when the concentration of ICA was 1 × 10− 6 mol/L. Conclusion: All the above results implied that ICA could significantly increase proliferation and enhance the osteogenic differentiation of hAMSCs, especially when the concentration of ICA was 1 × 10− 6 mol/L. Keywords: Human amniotic mesenchymal stem cell, Icariin, Proliferation, Osteogenic differentiation
Background Bone defects/losses is a serious problem in orthopedics due to low healing rate using traditional treatment and increases patients’ pain and heavily burdens families [1, 2]. Over the last two decades, tissue engineering technology has been applied extensively for clinical research. For example, stem cellbased tissue regeneration showed certain curative effects as a novel curative therapy for bone diseases. However, methods for collection, isolation, and maintenance of stem cells in vitro is still a challenge of stem cell-based clinical healing * Correspondence: [email protected] † Fang Wang and Zhiyong Yang contributed equally to this work. 1 Oral Maxillofacia Trauma and Orthognathic Surgery, Hospital of Stomatology, Zunyi Medical University, Zunyi, Guizhou, China Full list of author information is available at the end of the article
for bone disorder, which is also disadvantageous to reduce operation time [3]. Moreover, it is still a worry whether stem cell would differentiate into bone-forming osteoblasts. Although the osteogenic inducers such as bone morphogenetic proteins (BMP) and vascular endothelial growth factor (VEGF) are important factors for healing of bone defects/ losses, there are still some disadvantages for low differentiation efficiency, complicated formulas, and high cost [4]
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