ATF5 involved in radioresistance in nasopharyngeal carcinoma by promoting epithelial-to-mesenchymal phenotype transition
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ATF5 involved in radioresistance in nasopharyngeal carcinoma by promoting epithelial‑to‑mesenchymal phenotype transition Yu Shuai1 · Erxi Fan1 · Qiuyue Zhong1 · Guangyong Feng1 · Qiying Chen1 · Xiaoxia Gou1 · Guihai Zhang1,2 Received: 29 December 2019 / Accepted: 18 April 2020 © The Author(s) 2020
Abstract Purpose This study aimed to investigate the effects of activating transcription factor-5 (ATF5) on nasopharyngeal carcinoma (NPC) cell radioresistance. Methods HONE-1 nasopharyngeal carcinoma cells were irradiated by conventional fractionation to generate HONE-1R radiotherapy-resistant cells. Short hairpin RNA (shRNA) expression plasmids targeting the ATF5 gene were constructed and transfected into the HONE-1R cell line. The proliferation assay, colony formation analysis, Transwell Boyden chamber assay and other experimental methods were performed to verify changes in the radiosensitivity and other biological of NPC cells. Results X-ray irradiation significantly promoted the upregulation of ATF5 protein levels in HONE-1 cells, and the protein expression of ATF5 increased with the dose of X-ray irradiation (p
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