Bacillus amyloliquefaciens alters the diversity of volatile and non-volatile metabolites and induces the expression of d
- PDF / 1,404,950 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 71 Downloads / 152 Views
ORIGINAL ARTICLE
Bacillus amyloliquefaciens alters the diversity of volatile and non-volatile metabolites and induces the expression of defence genes for the management of Botrytis leaf blight of Lilium under protected conditions S. Nakkeeran 1 & R. Priyanka 1 & S. Rajamanickam 1 & U. Sivakumar 2 Received: 4 October 2019 / Accepted: 24 June 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract Lilium leaf blight induced by Botrytis cinerea is a major threat in the cultivation of Lilium in the Nilgiris province of Tamil Nadu, India under protected conditions. The present study aims to understand bipartite and tripartite interactions between B. cinerea, Lilium and B. amyloliquefaciens to develop a cost-effective antagonist to manage Botrytis leaf blight. The in vitro antagonism by B. amyloliquefaciens (VB7) suppressed the mycelial growth of B. cinerea by up to 46%. Foliar application of B. amyloliquefaciens (VB7) colonized the phylloplane within 48 h and prevented conidial germination of B. cinerea. Biofilm on the leaf surface colonized the conidia of B. cinerea (SEL). The colonized conidia were parasitized by bacterial cells, resulting in shrinkage. The bipartite interaction between B. amyloliquefaciens (VB7) and mycelial biomass of B. cinerea as sole carbon source produced volatile and non-volatile antifungal compounds. Tripartite interactions between Lilium leaf, B. amyloliquefaciens (VB7) and conidia of B. cinerea produced five different non-volatile antifungal and antibacterial compounds. Expression of defence genes through qRT-PCR analysis indicated that the transcript level of PAL, PR 10 and ascorbate peroxidase (APX) were triggered in Lilium plants treated with B. amyloliquefaciens (VB7), challenged against B. cinerea (SEL). Bulb dipping and soil drenching along with foliar application suppressed Botrytis leaf blight and increased Lilium stem yield under protected conditions. Keywords Leaf blight of Lilium . Bacillus . Antifungal metabolites . ESEM . Defence genes
Introduction Lilium cut flowers are cultivated for their scented nature and attractive colours. Lilium comprises around 100 species distributed throughout the temperate regions across the globe (Siljak-Yakovlev et al. 2003). In the northern parts of India, it is cultivated in Himachal Pradesh and Uttaranchal. In the
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42161-020-00602-6) contains supplementary material, which is available to authorized users. * S. Nakkeeran [email protected]; [email protected] 1
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
2
Department of Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India
southern part, it is cultivated in the Nilgiris province of Tamil Nadu. The crop is infected by leaf blight pathogens caused by Botrytis cinerea, an emerging disease in India (Dhyani et al. 2012). Botrytis leaf blight destroyed 15% of the plants under greenhouse condition and cause
Data Loading...
