Beyond host specificity: the biotechnological exploitation of chitolectin from teratocytes of Toxoneuron nigriceps to co
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ORIGINAL PAPER
Beyond host specificity: the biotechnological exploitation of chitolectin from teratocytes of Toxoneuron nigriceps to control non‑permissive hosts Bruna Laís Merlin1 · Lilian Ellen Pino2,3 · Lázaro Eustáquio Pereira Peres3 · Fábio Prataviera4 · Edwin Moises Marcos Ortega4 · Fernando Luis Cônsoli1 Received: 21 May 2020 / Revised: 21 September 2020 / Accepted: 25 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The virulence factors (VF) that parasitoids use to regulate the growth and development of their hosts have potential as new events in transgenic plants. VFs are promising candidates for biotechnological exploitation and implementation of pestcontrol tools to meet the world’s demand for food in 2100, while sustainably increasing agricultural production. However, VFs have not been used in commercial transgenic plants, since they are thought to target only a narrow range of insects, due to the parasitoid host specialization. We investigated the potential of chitolectin (TnChit), produced by the teratocytes of Toxoneuron nigriceps (Viereck) (Hymenoptera: Braconidae), as a transgenic event to control several non-permissive hosts: Chrysodeixis includens (Walker), Spodoptera albula (Walker), Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) and Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Third instars of C. includens, S. albula and S. frugiperda had lower survival and probability of molting on TnChit-modified plants than on control plants. The nutritional indices of S. frugiperda were also altered. Larval survival and pupal weight of males of T. absoluta were reduced on transgenic plants. Adult emergence of B. tabaci was also reduced. The expression of the TnChit gene differed in the transformation events obtained and was consistent with the biological effects observed. The effects of TnChit on non-permissive hosts demonstrate the potential of the virulence factors that parasitoids employ in host regulation, as a source of new genes in plant transformation for control of insect pests. Keywords Biotechnology · Host–parasitoid interactions · Resilient agricultural systems · Sustainable pest management · Transgenic plants
Key message • Parasitoids utilize molecules to regulate their host’s physCommunicated by Antonio Biondi. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10340-020-01290-y) contains supplementary material, which is available to authorized users. * Fernando Luis Cônsoli [email protected] 1
2
Insect Interactions Laboratory, Department of Entomology and Acarology, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil Plant Breeding Laboratory, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP, Brazil
iology and development.
• Despite their potential for biotechnological exploitation
for insect pest control, there is limited knowledge on the efficacy of such m
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