Bifidobacteria adolescentis regulated immune responses and gut microbial composition to alleviate DNFB-induced atopic de
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ORIGINAL CONTRIBUTION
Bifidobacteria adolescentis regulated immune responses and gut microbial composition to alleviate DNFB‑induced atopic dermatitis in mice Zhifeng Fang1,2 · Lingzhi Li1,2 · Jianxian Zhao1,2,5 · Hao Zhang1,2,3,4,5 · Yuan‑Kun Lee6 · Wenwei Lu1,2,3,5 · Wei Chen1,2,3,7 Received: 3 September 2019 / Accepted: 11 November 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract Purpose Emerging studies have reported gut microbial composition plays a key role in alleviating AD clinical symptoms during the probiotic intervention, but the correlation among clinical symptoms, immune responses and gut microbial alteration needs to be explored. Therefore, the objective was to investigate the correlation during Bifidobacterium adolescentis intervention in DNFB-induced AD mice. Methods The mice were randomly divided into nine groups and fed B. adolescentis for 3 weeks. At the end of the experiment, clinical and immune indicators were assessed. Flow cytometry was performed to explore the effect of B. adolescentis on regulatory T cells in the spleen. V3–V4 region of the 16S ribosomal RNA (rRNA) gene was sequenced to evaluate changes in the gut microbiota. Results Bifidobacteria adolescentis treatments reduced ear and skin thickness and suppressed eosinophils and mast cells infiltration. Th1- and Th2-type responses were regulated and the Tregs population was promoted in the spleen by B. adolescentis treatments. Bifidobacteria adolescentis increased the relative abundance of Lactobacillus but decrease Dorea and Pediococcus. Propionic and butyric acids were increased but isovaleric acid was decreased by B. adolescentis treatment. Besides, the functional modules, such as fatty acid biosynthesis, antigen processing and presentation were upregulated by B. adolescentis Ad1 treatment compared to the DNFB group. Conclusion Collectively, these results imply that B. adolescentis with the role of immunomodulation promotes Treg differentiation and suppresses Th2 responses, and increases the proportion of Lactobacillus that is positively correlated to increase in propionic acid production, and thus has the potential for AD amelioration. Keywords Bifidobacterium adolescentis · Immune responses · Gut microbiota · Short-chain fatty acids · Atopic dermatitis
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00394-019-02145-8) contains supplementary material, which is available to authorized users. * Wenwei Lu [email protected] 1
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2
School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
3
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, Jiangsu, China
4
Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institut Wuxi Branch, Wuxi 214122, Jiangsu, China
5
(Yangzhou) Institute of Food Biotechnology, Jiangnan University, Y
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