Biodegradation of acephate by Bacillus paramycoides NDZ and its degradation pathway
- PDF / 2,618,437 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 93 Downloads / 185 Views
(2020) 36:155
ORIGINAL PAPER
Biodegradation of acephate by Bacillus paramycoides NDZ and its degradation pathway Jianjun Ren1,2 · Chongqing Wang3 · Huhetaoli1,2 · Chunyu Li1,2 · Bo Fan4 · Dongze Niu1,2 Received: 22 December 2019 / Accepted: 14 September 2020 © Springer Nature B.V. 2020
Abstract Acephate is widely used in agriculture, but its poisonous metabolites and poor sorption characteristics make it a serious environmental pollutant and toxicant to human health. To screen novel bacteria for biodegradation of acephate and uncover its degradation pathway, a strain called NDZ that is capable of utilizing acephate as a sole carbon and energy source was isolated from severely contaminated cultivated land. The bacterium was identified as Bacillus paramycoides based on 16S rDNA sequence analyses. The growth and degradation capacities of B. paramycoides NDZ under different conditions were studied using optical density at 600 nm (OD600) and high-performance liquid chromatography (HPLC). The results showed that B. paramycoides NDZ can grow well with acephate as its sole carbon source (OD600 = 0.76), and degraded about 76% of acephate in mineral salt medium with an initial concentration of 500 mg/L within 48 h. The results of response surface methodology revealed the optimal conditions for degradation was 36 ℃ and pH 6.85 with 526 mg/L acephate. Gas chromatography–mass spectrometry showed that methamidophos was the main metabolite of B. paramycoides NDZ, different from the degradation products of high-temperature steam (121 °C, 103 kPa). Based on the detection of this intermediate, we inferred that acephate was degraded to methamidophos through hydrolysis of the amide linkage, after which methamidophos was degraded to some small molecules, which can be metabolized easily by the bacterium. In summary, B. paramycoides NDZ is a potentially useful bacterium for acephate degradation and remediation of contaminated soils.
* Dongze Niu [email protected] 1
Institute of Urban and Rural Mining, Changzhou University, Changzhou 213164, China
2
National‑Local Joint Engineering Research Center for Biomass Refining and High‑Quality Utilization, Changzhou University, Changzhou 213164, China
3
Beijing Monitoring Station for Animal Husbandry Environment, Beijing 102200, China
4
School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China
13
Vol.:(0123456789)
155
Page 2 of 11
World Journal of Microbiology and Biotechnology
(2020) 36:155
Graphic abstract
Keywords Acephate · Biodegradation · Methamidophos · Pathway · Bacillus paramycoides
Introduction Pesticides are crucial to modern agriculture around the world. The global use of pesticides for agriculture was about 4,113,591 tons in 2017 (FAO 2019). Using pesticides, farmers can reduce labor and ensure maximum crop yields. However, more than 85% of sprayed chemicals fail to reach their target pests or weeds due to extensive and indiscriminate usage (Jiang et al. 2018). Residual xenobiotics and their metabolites in
Data Loading...
