Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency
- PDF / 2,969,725 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 92 Downloads / 232 Views
ORIGINAL ARTICLE
Annals of Microbiology
Open Access
Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency Xiujie Gong1,2, Hongtao Zou1*, Chunrong Qian2, Yang Yu2, Yubo Hao2, Liang Li2, Qiuju Wang3, Yubo Jiang2 and Juntao Ma4
Abstract Purpose: The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization. Methods: According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Result: The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain Streptomyces sp. G1T has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain Streptomyces sp. G2T has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain Streptomyces sp. G3T to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Conclusion: Streptomyces sp. G1T, Streptomyces sp. G2T, and Streptomyces sp. G3T screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources. Keywords: Corn straw, In situ degrading bacteria, Strain identification, Flora construction, Degradation efficiency
* Correspondence: [email protected] 1 College of Land and Environment, Shenyang Agricultural University, No. 120, DongLing Road, Shenhe District, Shenyang 110866, People’s Republic of China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution
Data Loading...
