Biodegradation of Reactive Orange 16 Dye in Microbial Fuel Cell: An Innovative Way to Minimize Waste Along with Electric
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Biodegradation of Reactive Orange 16 Dye in Microbial Fuel Cell: An Innovative Way to Minimize Waste Along with Electricity Production Amrita Shahi 1 & Birendra Nath Rai 1 & Ram Sharan Singh 1 Received: 26 June 2019 / Accepted: 12 March 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Microbial fuel cell (MFC) is an attractive green technology which harnesses the power of microorganism for the production of electricity along with bioremediation of waste. However, the bioremediation of the high concentration of dye wastewater in MFC remains unclear. In present study, double-chambered MFC inoculated with mixed bacterial consortium was used for bioremediation of reactive orange 16 (RO-16) dyes at a very high and variable concentration range of 100 to 1000 ppm. Maximum voltage was obtained for 100 ppm of dye and was found to be 0.5791 V along with a power density of 0.0851 W/m3. Till 500 ppm concentration of dye COD removal efficiency remains in range of 40 to 100% thereafter it decreases. The maximum concentration of CO2 was found to be 2% at 1000 ppm which confirms the biodegradation phenomena in MFC. Kinetics of biodegradation of reactive orange 16 were studied using Haldane inhibitory kinetic model and kinetic constants μmax, Ks, and Ki were calculated and found to be 0.417 day−1, 206.2 ppm, and 447.12 ppm respectively. The experimental results showed inhibitory condition in the MFC after 500 ppm and it was supported by the value of inhibitory kinetic constant Ki = 447.12 ppm. This study opened the possibility of bioremediation of dyes at high concentrations in MFCs. Keywords Haldane kinetics . COD removal efficiency . Microbial fuel cell (MFC) . Reactive Orange 16 Highlights • First-time reactive orange 16 dye was taken as a substrate for the microbial fuel cells. • Almost 100% color removal was observed at 100 ppm of dye within 24 h. • The high concentration of dye (500 ppm) is successfully removed in MFC in 24 h. • If the residence time of dye is increased from 24 to 72 h then 600 to 800 ppm of dye can also be removed. • The inhibition study was done with the Haldane model and kinetic constants were calculated and supported the experimental observations. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12010-02003306-w) contains supplementary material, which is available to authorized users.
* Ram Sharan Singh [email protected]; [email protected] Extended author information available on the last page of the article
Applied Biochemistry and Biotechnology
Introduction The use of synthetic dye increases day by day due to its low cost and ease of availability. Nowadays, 700,000 tons of synthetic dyes are used by the industries and about 5–10% left as residue are discharged into water bodies [1–4]. Discharge of even a very small amount of dye (10–50 mg/L) not only esthetically unacceptable but it can also cause serious threats to human as well as aquatic life. Some dyes are reported to be carcinogens and may cause skin ca
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