Biophysical Chemistry of Proteins An Introduction to Laboratory Meth
Undergraduate biochemistry courses cover what proteins do, as enzymes, receptors, hormones, motors or structural components. The much more interesting question is how can proteins achieve all these functions? Presented here is an overview of the methods u
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Engelbert Buxbaum
Biophysical Chemistry of Proteins An Introduction to Laboratory Methods
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Engelbert Buxbaum Ross University School of Medicine P.O. Box 266 Biochemistry Portsmouth Campus 266 Roseau Dominica engelbert [email protected]
ISBN 978-1-4419-7250-7 e-ISBN 978-1-4419-7251-4 DOI 10.1007/978-1-4419-7251-4 Springer New York Dordrecht Heidelberg London © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
During undergraduate courses in biochemistry you learned what proteins do as enzymes, receptors, hormones, motors or structural components. The more interesting question, how proteins can achieve all these functions, is usually asked only in graduate courses, and in many cases it is a topic of ongoing research. Here I want to present an overview of the methods used in such research projects, their possible applications, and their limitations. I have limited the presentation to a level where a general background in chemistry, physics, and mathematics is sufficient to follow the discussion. Quantum mechanics, where required, is treated in a purely qualitative manner. A good understanding of protein structure and enzymology is required, but these topics I have covered in a separate volume [44]. Apart from graduate training in protein science this book should also be useful as a reference for people who work with proteins. After studying this book you should be able to collaborate with workers who have the required instruments and use these methods routinely. You should also be able to understand papers which make use of such methods. However, before embarking on independent research using these methods you are directed to the literature cited for a more in-depth, more quantitative coverage. This book focuses on the biophysical chemistry of proteins. The use of nucleic acid-based methods [360], although in many cases very relevant and informative, is outside the scope of this text. Also only hinted at are modern approaches to computational biochemistry [20, 180, 231]. In the end, the models derived from such techniques have to be verified by experiments. If this book stimulates such studies, it has served its purpose.
Acknowledgements I wish to thank all my students, friends, and colleagues who
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