Bixin loaded on polymeric nanoparticles: synthesis, characterization, and antioxidant applications in a biological syste
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ORIGINAL ARTICLE
Bixin loaded on polymeric nanoparticles: synthesis, characterization, and antioxidant applications in a biological system Alexsandro Tavares Figueiredo‑Junior1,4 · Francisca de Fátima dos Anjos2 · Francisco das Chagas de Melo Brito3 · Vicente Galber Freitas Viana3 · Samuel Santos Valença1 · Manuella Lanzetti1 · Priscilla Vanessa Finotelli4 Received: 10 June 2020 / Accepted: 3 September 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Acute lung inflammation (ALI) is a disease caused by many factors including cigarette smoke (CS) exposure. Natural products have demonstrated interesting antioxidant and anti-inflammatory activities able to prevent the damage caused in the lungs by CS. Annatto seed alkaline extract is rich in a pigment called bixin, which has a potent antioxidant effect but is too poorly hydrophilic, making it difficult to use in oral treatments and putting it as an attractive molecule for been loaded on polymeric nanoparticles. Therefore, in this study, we aimed to develop and characterize nanoparticles of bixin (npBX) and to conduct initial tests in vivo with oral treatment of ALI induced by CS in C57BL/6 mice with npBX. Bixin was purified from annatto seeds with a high degree of purity and rigorously characterized. Nanoparticles were developed using the interfacial deposition method and showed a mean hydrodynamic diameter of 23.7 ± 0.2 nm, polydispersity index of 0.226 ± 0.006, zeta potential of − 21.69 ± 9.90 mV, and drug loading of approximately 24.23 ± 0.41 mg/g. Treatment with npBX prevented increases in superoxide anion and total bronchoalveolar lavage leukocyte levels in mice exposed to CS. These data provide support for npBX as a promising therapeutic tool for lung diseases. Keywords Bixin · Polymeric nanoparticles · Polycaprolactone · Drug delivery · Lung inflammation · Cigarette smoke
Introduction Bixa orellana L., popularly known as annatto, is a plant native to tropical areas of the Americas, from the Bixaceae family, and it contains many carotenoid derivatives, terpenoids, tocotrienols and flavonoids in its seed composition (Rodrigues et al. 2007). Bixa orellana L. seed extracts are widely used as a natural coloring for food and by the cosmetics industry.
* Priscilla Vanessa Finotelli [email protected] 1
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
2
Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil
3
Federal Institute of Piaui, Teresina, PI, Brazil
4
Departamento de Produtos Naturais e Alimentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Bloco A, 2° andar, sala 38, Rio de Janeiro 21941‑590, Brazil
Annatto seed extract has been targeted for the treatment and prevention of some health disorders in animal models, such as low-density lipoprotein (LDL) oxidation (Conte et al. 2019), hepatocarcinoma (Kumar et al. 2018), diabetes (Pinzon-Garcia et al. 2018), skin problems (Rojo de la Vega et
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