Broad-scale detection of environmental DNA for an invasive macrophyte and the relationship between DNA concentration and
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ORIGINAL PAPER
Broad-scale detection of environmental DNA for an invasive macrophyte and the relationship between DNA concentration and coverage in rivers Hideyuki Doi . Yoshihisa Akamatsu . Masuji Goto . Ryutei Inui . Takashi Komuro . Mariko Nagano . Toshifumi Minamoto
Received: 11 September 2019 / Accepted: 29 September 2020 Springer Nature Switzerland AG 2020
Abstract Environmental DNA (eDNA) techniques have the potential to become useful tools for investigating the distribution and biomass of aquatic organisms. Additionally, Unmanned Aerial Vehicle (UAV) photos have recently been applied to evaluate vegetation cover. We tested the ability of eDNA techniques to evaluate the distribution and biomass of an invasive macrophyte, Egeria densa (Brazilian waterweed). We conducted field surveys in two Japanese rivers to determine the abundance of eDNA Hideyuki Doi and Yoshihisa Akamatsu contributed equally.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10530-020-02380-9) contains supplementary material, which is available to authorized users.
from E. densa and compared it with macrophyte coverage by evaluating UAV photographs taken in the summer and winter. We found eDNA of E. densa in all sampled sites, including various sites with no macrophyte coverage, indicating that there was a broad-scale distribution of E. densa eDNA. In summer, we detected a slightly positive relationship between E. densa eDNA and macrophyte coverage. We did not find a clear relationship between E. densa eDNA and macrophyte coverage, probably because of their widespread and dense distribution and the flow of their materials in the rivers. The seasonal differences in the regression may have been caused by the seasonal growing period of E. densa in the summer, with a decline in their biomass in late autumn and winter. We confirmed the ability of eDNA techniques
H. Doi (&) M. Nagano Graduate School of Simulation Studies, University of Hyogo, Kobe, Japan e-mail: [email protected]
T. Komuro National Institute of Maritime, Port and Aviation Technology, Port and Airport Research Institute, Yokosuka, Japan
Y. Akamatsu M. Goto R. Inui T. Komuro Graduate School of Science and Engineering, Yamaguchi University, Ube, Japan
T. Minamoto Graduate School of Human Development and Environment, Kobe University, Kobe, Japan
M. Goto Nippon Koei Co., Ltd., Tsukuba, Japan R. Inui Faculty of Socio-Environmental Studies, Fukuoka Institute of Technology, Fukuoka, Japan
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to survey this invasive macrophyte in rivers by comparing results with coverage measurements from UAV photographs.
Fig. 1 Map of Takatsu River and Saba River. The gray and c white basins are the Takatsu and Saba rivers, respectively. Stream and coastline data were from https://nlftp.mlit.go.jp/ksj/, the National Land Information Division, Ministry of Land, Infrastructure, Transport and Tourism of Japan
Keywords eDNA Invasive macrophyte River Quantitative P
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