Burkholderia sp. Strain AU4i Promotes Arabidopsis Growth and Increases Plant Defence Response to Necrotrophic Pathogens
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Burkholderia sp. Strain AU4i Promotes Arabidopsis Growth and Increases Plant Defence Response to Necrotrophic Pathogens María Belén Colavolpe1 · Natalia Marina Villarreal1 · Silvia Estefanía Langer1 · Fernando Matías Romero1 · Gustavo Adolfo Martínez2,3 · Adesh Saini4 · Oscar Adolfo Ruiz1 · María Marina1 Received: 16 January 2020 / Accepted: 29 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Burkholderia sp. AU4i is a strain previously isolated from pea, which can act as plant growth-promoting rhizobacteria (PGPR). Here, we demonstrate that AU4i is able to promote Arabidopsis growth and increase the number of lateral roots as well as shoot and root dry weight. The inoculation of Arabidopsis leaves with AU4i significantly reduced the lesion’s areas provoked by the necrotrophic pathogens Botrytis cinerea and Sclerotinia sclerotiorum. Bacterial inoculation induced the expression of the jasmonate responsive marker gene AtPDF1.2, but not the expression of salicylic acid marker gene AtPR-1. Notwithstanding, similar AU4i protective effect was observed in Arabidopsis mutants coi1 and sid2-2 (defective in jasmonate perception and salicylic acid synthesis, respectively) compared with Col-0 plants. Thus, the independence of the AU4i protective effect and plant defence signalling pathways is suggested. AU4i inoculation was able to induced anthocyanins and phenolic compounds accumulation, as well as phenylalanine ammonia-lyase activity. Moreover, the effect of AU4i inoculation on the metabolism of plant cell wall was evaluated. As a result, B. cinerea and S. sclerotiorum mycelial growth was reduced in culture media containing cell wall polysaccharides from inoculated Arabidopsis leaves. Additionally, leaves colonized by AU4i showed the downregulation of genes involved in hemicelluloses and pectins degradation (AtExp8 and AtAra1, respectively) and the upregulation of AtPME3, a gene encoding a putative pectin methylesterase involved in pectin main chain de-esterification. Taken together, our results suggest Burkholderia sp. AU4i not only as potential biocontrol agent, but also as a good candidate for better understanding the mechanisms used by PGPRs to protect plants from fungal attacks. Keywords Burkholderia · Botrytis cinerea · Sclerotinia sclerotiorum · Plant cell wall · Plant defence · Plant growth promotion
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10238-6) contains supplementary material, which is available to authorized users. * María Marina [email protected] 1
Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas Y Técnicas (INTECH, UNSAM-CONICET), 7130 Chascomús, Buenos Aires, Argentina
2
Instituto de Fisiología Vegetal (INFIVE, CONICET-UNLP), La Plata, Buenos Aires, Argentina
3
Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), La Plata, Buenos Aires, Argentina
4
Faculty of Applied Scien
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