C 60 in olive oil causes light-dependent toxicity and does not extend lifespan in mice
- PDF / 579,363 Bytes
- 13 Pages / 547.087 x 737.008 pts Page_size
- 94 Downloads / 227 Views
ORIGINAL ARTICLE
C60 in olive oil causes light-dependent toxicity and does not extend lifespan in mice Kristopher J. Grohn & Brandon S. Moyer & Danique C. Wortel & Cheyanne M. Fisher & Ellie Lumen & Anthony H. Bianchi & Kathleen Kelly & Paul S. Campbell & Douglas E. Hagrman & Roger G. Bagg & James Clement & Aaron J. Wolfe & Andrea Basso & Cristina Nicoletti & Giovanni Lai & Mauro Provinciali & Marco Malavolta & Kelsey J. Moody
Received: 8 March 2020 / Accepted: 22 October 2020 # American Aging Association 2020
Abstract C60 is a potent antioxidant that has been reported to substantially extend the lifespan of rodents when formulated in olive oil (C60-OO) or extra virgin Kelsey J. Moody and Marco Malavolta shared senior authorship. Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007 /s11357-020-00292-z. K. J. Grohn : B. S. Moyer : D. C. Wortel : C. M. Fisher : E. Lumen : A. H. Bianchi : K. Kelly : P. S. Campbell : A. J. Wolfe : K. J. Moody Ichor Therapeutics, Inc., 2521 US Route 11, LaFayette, NY 13084, USA K. J. Grohn Department of Chemistry, College of Environmental Science and Forestry, State University of New York, Syracuse, NY 13210, USA E. Lumen : J. Clement Betterhumans Inc., Gainesville, FL, USA D. E. Hagrman Department of Chemistry and Physical Sciences, State University of New York, Onondaga Community College, Syracuse, NY 13215, USA R. G. Bagg BioSenex, Ltd., Lyndhurst, 1 Cranmer Street, Nottingham, Nottinghamshire NG10 1NJ, UK A. Basso : C. Nicoletti : G. Lai : M. Provinciali : M. Malavolta (*) Advanced Technology Center for Aging Research, IRCCS INRCA, via Birarelli 8, 60121 Ancona, Italy e-mail: [email protected]
olive oil (C60-EVOO). Despite there being no regulated form of C60-OO, people have begun obtaining it from online sources and dosing it to themselves or their pets, presumably with the assumption of safety and efficacy. In this study, we obtain C60-OO from a sample of online vendors, and find marked discrepancies in appearance, impurity profile, concentration, and activity relative to pristine C60-OO formulated in-house. We additionally find that pristine C60-OO causes no acute toxicity in a rodent model but does form toxic species that can cause significant morbidity and mortality in mice in under 2 weeks when exposed to light levels consistent with ambient light. Intraperitoneal injections of C60-OO did not affect the lifespan of CB6F1 female mice. Finally, we conduct a lifespan and health span study in males and females C57BL/6 J mice comparing oral treatment with pristine C60-EVOO and EVOO alone versus untreated controls. We failed to observe significant lifespan and health span benefits of C60-EVOO or EVOO supplementation compared to untreated controls, both starting the treatment in adult or old age. Our results call into question the biological benefit of C60-OO in aging. Keywords Antioxidant; . C60-OO; . Fullerene
Introduction Since its discovery in 1985, C60 fullerene has captured the attention of chemists and biolog
Data Loading...
