Synthesized chrysin-loaded nanoliposomes improves cadmium-induced toxicity in mice
- PDF / 3,487,945 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 89 Downloads / 198 Views
RESEARCH ARTICLE
Synthesized chrysin-loaded nanoliposomes improves cadmium-induced toxicity in mice Mahsan Beyrami 1 & Ehsan Karimi 1
&
Ehsan Oskoueian 2
Received: 23 January 2020 / Accepted: 13 July 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In this study, chrysin as a natural flavonoid was encapsulated in nanoliposomal structures, and the synthesized nanoliposomeloaded chrysin (NLC) was further characterized for its physical properties and cytoprotective effects in mice that received cadmium-containing water. The results showed that the synthesized NLC is possessed spherical structure with the size of 185.1 nm and negative surface charge of − 26 mV with a poly dispersity index of 0.26. The mice received cadmium (2 mg/kg body weight/day) through drinking water showed weight loss and decease in the feed intake significantly (p ≤ 0.05). The cadmium notably (p ≤ 0.05) increased the liver enzymes including aspartate aminotransferase, alanine transaminase, and alkaline phosphatase; altered the liver metal deposition (cadmium, copper, manganese, selenium, and zinc); and induced hepatic oxidative stress (inducible nitric oxide synthase, catalase, superoxide dismutase, and glutathione peroxidase genes) with no remarkable histopathological changes. Furthermore, the cadmium impaired the morphology of jejunum through reducing villus height and villus width and increasing the crypt depth. Providing NLC as a dietary supplement at the concentrations of 2.5 and 5 mg/kg mice body weight significantly (p ≤ 0.05) improved the feed intake and body weight gain, modulated the liver enzymes, and alleviated the hepatic oxidative stress. The NLC also improved the antioxidant mineral deposition in the liver and morphohistological structure of jejunum. Consequently, the NLC is suggested as a potential dietary supplement to alleviate the symptoms of cadmium-induced toxicity in mice. Keywords Antioxidant activity . Cadmium-mediated cytotoxic impacts . Cytoprotective . Gene expression . Nanoliposome-loaded chrysin (NLC)
Introduction Metals can enter the body homeostasis and may indicate toxic effects. Heavy metals can move structural metals from their functional sites and lead to malfunctioning of the various Mahsan Beyrami, Ehsan Karimi and Ehsan Oskoueian contributed equally to this work. Responsible editor: Mohamed M. Abdel-Daim * Ehsan Karimi [email protected] * Ehsan Oskoueian [email protected] 1
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2
Mashhad Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Mashhad, Iran
enzymes. Binding heavy metals to biomolecules such as DNA and proteins have been found as a cause of molecular oxidative damage (Flora et al. 2008). The soil, water, air and even food are considered the main sources of cadmium (Cd2+), which can influence human health. Heavy metals are able to induce oxidative stress and show cytotoxic activity due to t
Data Loading...
