Cell-Based Microarrays Review of Applications, Developments and Tech
This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays. Since their development in 2001, cell-based microarrays have advanced significantly to include
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Ella Palmer
Cell-Based Microarrays Review of Applications, Developments and Technological Advances 123
SpringerBriefs in Cell Biology
For further volumes: http://www.springer.com/series/10708
Ella Palmer
Cell-Based Microarrays Review of Applications, Developments and Technological Advances
Ella Palmer Faculty of Medicine, Quantitative Systems Biology MRC Clinical Sciences Centre Imperial College London London, UK
ISBN 978-1-4939-0593-5 ISBN 978-1-4939-0594-2 (eBook) DOI 10.1007/978-1-4939-0594-2 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014934131 © Ella Palmer 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
Cell-based microarrays are a technique originally described by the Sabatini group in 2001 as “reverse transfection.” cDNA or siRNAs in vector constructs are printed onto a coated glass slide using a robotic microarrayer and the vector constructs are transfected in defined areas within cells grown over the surface of the slide or microplate. Downstream functional assays are then undertaken on the cells. Cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over-expression and knock down of proteins. Since their development in 2001, they have advanced sig
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