Protein Microarrays Methods and Protocols
Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of
- PDF / 12,426,341 Bytes
- 393 Pages / 504.567 x 720 pts Page_size
- 65 Downloads / 236 Views
in
Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
wwwwwwwwwwwwwww
Protein Microarrays Methods and Protocols Edited by
Ulrike Korf Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany
Editor Ulrike Korf Division of Molecular Genome Analysis German Cancer Research Center (DKFZ) Heidelberg, Germany [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-285-4 e-ISBN 978-1-61779-286-1 DOI 10.1007/978-1-61779-286-1 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011934254 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Proteins are involved in almost any aspect of cellular function. The cellular proteome is subjected to a steady flow of dynamic changes, and therefore is a very suitable readout for the functional properties of a cell or an organism. Proteins, for example, build the cellular architecture, and are essential components of membranous compartments confining a cell, as well as subcellular organelles. Networks of tightly regulated enzymes are in command of the energy supply, and provide molecular building blocks, such as carbohydrates, lipids, and nucleic acids. Other proteins are involved in replication and transcriptional processes, and assist in the translation of new proteins. Proteins in extracellular fluids maintain the communication between cells of a tissue as well as within an organism and may serve as disease biomarkers. The number of different proteins encoded by the genome is increased by at least an order of magnitude, due to the introduction of posttranslational modifications, such as glycosylation, lipid-modifications, acetylation, and by protein phosphorylation which is the best-studied mode of cellular regulation. Understanding protein function and the regulation of signaling networks requires large-scale efforts which enable the dynamic analysis of numerous samples in parallel. Progress in functional proteomics has been limited for a long time, partially because of limitations in assay sensitivity a
Data Loading...
