Cell Self Assembly of Intracellular Interface Using Cell Migration
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Cell Self Assembly of Intracellular Interface Using Cell Migration Takayuki Hoshino1, Tomohiro Konno2, Kazuhiko Ishihara2, and Keisuke Morishima3 1 Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 224-16,Naka-cho, Koganei-shi, Tokyo, 184-8588, Japan 2 Department of Materials Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan 3 Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 224-16, Naka-cho, Koganei-shi, Tokyo, 184-8588, Japan ABSTRACT A cell-driven self-assembly of intracellular nano-device was proposed for bio-hybrid interface. This cells-driven self-assembly employed cell migration force to insert a conductive nanoneedle which would be worked as intracellular electrode. Such a nanoneedle was fabricated in the bottom of a microwell using focused ion beam induce deposition. The microwell structure with a coating of cell adhesion molecules was employed as the scaffold of the cell migration. A glass plate with the microwells had a non cell binding coating of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer as an anti-biofouling material. Thus a cell adhered only on the wall of a microwell then the cell migrated into the microwell. Adhesion force and migration force induced self-insertion of the nanoneedle into a live cell body using the cell’s own migration force. The inserted nanoneedle was made of electrical conductive tungsten, so the intracellular nanoneedle might extract intracellular potential more precisely than extracellular electrode, while inducing much less damage to cells. In the future, the technique of cell-driven self-insertion of nanoneedle may be integrated with multi electrode arrays for developing long-lasting measurements device on cellular network researches, or the risk assessment of the nanomaterials on cellular activities.
INTRODUCTION This paper reports an assembly method of an intracellular interface between a nano structure and a living cell. We proposed an intracellular nanodevice assembly using cells’ own mechanisms. To achieve this concept, we focused on cell migration on an extra cellular matrix for a driving force of the self-assembly intracellular probing. In earlier studies, the migration and adhesion forces of cells on the surface of a culture plate were reported as much greater than the insertion force of a nanoneedle [1, 2]. In this study, we describe an intracellular nanoneedle inserted by the cell migration force toward developing intracellular recording devices. Figure 1 describes the conceptual illustration of the cell-driven self-insertion of nanoneedle using cell’s own migration force. Here we designed cell-driven self-insertion which controlled by patterned extra cellular matrix.
force of migration force for insertion extr a ce llul ar m atri x
Figure 1. Fundamental concept of cell-driven self-assembly of an intracellular device. The force of cell migration on the extra cellular matrix is much greater than the insertion force of a nanonee
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