Changes in the microbial community of Litopenaeus vannamei larvae and rearing water during different growth stages after
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Changes in the microbial community of Litopenaeus vannamei larvae and rearing water during different growth stages after disinfection treatment of hatchery water§ Yafei Duan1,2, Yapeng Tang1,2, Jianhua Huang1,2*, Jiasong Zhang1,2*, Heizhao Lin1,2, Shigui Jiang2, Ruixuan Wang2, and Guofu Wang3* 1
Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, P. R. China 2 Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, P. R. China 3 Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, P. R. China (Received Jan 31, 2020 / Revised Jun 4, 2020 / Accepted Jun 12, 2020)
Microbial communities greatly affect rearing water quality and the larvae health during shrimp hatchery periods. In this study, we investigated the microbial communities of rearing water and larvae of Litopenaeus vannamei after treating hatchery water with different kinds of chemical disinfectants: no disinfectants (Con), chlorine dioxide (ClO2), formaldehyde solution (HCHO), bleach powder (CaClO), and iodine (I2). The water and larval samples were collected from nauplius 6 (N6), zoea 1 (Z1), mysis 1 (M1), and postlarvae 1 (P1) shrimp growth periods. 16S rDNA high-throughput sequencing revealed that the bacterial composition of the rearing water was more complex than that of the larvae, and the bacterial community of the rearing water and the larvae fluctuated significantly at the P1 and Z1 periods, respectively. Disinfectants altered the bacterial diversity and composition of the rearing water and larvae. Specifically, in the rearing water of the P1 period, Proteobacteria abundance was increased in the HCHO group; while Bacteroidetes abundance was decreased in the ClO2, HCHO, and I2 groups but increased in the CaClO group. In the larvae of the Z1 period, Firmicutes (especially Bacillus class) abundance was increased in the CaClO group, but decreased in the ClO2, HCHO, and I2 groups. Network analyses revealed that the genera Donghicola, Roseibacterium, Candidatus-Cquiluna, and Nautella were enriched in the rearing water, while Halomonas, Vibrio, and Flavirhabdus had high abundance in the larvae. The survival of shrimp was influenced by disinfectants that were inconsistent with the bac*For correspondence. (J.H. Huang) E-mail: [email protected] / (J.S. Zhang) E-mail: [email protected] / (G.F. Wang) E-mail: [email protected] § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright ⓒ 2020, The Microbiological Society of Korea
terial community changes. These results will be helpful for using microbial characteristics to facilitate healthy shrimp nursery. Keywords: chemical disinfectants, growth stages, Litopenaeus vannamei larvae, microbial community, w
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