Changes of microbial diversity during pyrite bioleaching
- PDF / 973,905 Bytes
- 7 Pages / 595.22 x 842 pts (A4) Page_size
- 54 Downloads / 268 Views
Changes of microbial diversity during pyrite bioleaching YIN Lu(殷璐), YANG Hong-ying(杨洪英), LI Xiang(李想), TONG Lin-lin(佟琳琳), JIN Zhe-nan(金哲男), ZHANG Qin(张勤) School of Metallurgy, Northeastern University, Shenyang 110819, China © Central South University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract: Microorganisms, one of the key factors affecting the bioleaching process, change the components of extracellular polymeric substance (EPS) and community structure to survive in leaching environments. In this work, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD) and 16S rDNA high-throughput sequence analyses were used to reveal the microbial changes in planktonic and sessile phases during bioleaching. The results showed the occupation of sessile cells decreased from 66.2% to (10±3)%. After bioleaching, the planktonic and sessile cells have similar EPS, but they are different from the original cells. Pyrite dissolution mainly occurs at the early and late stages with the decreasing of particle diameter, by 50% and 40%, respectively. The 16S rDNA gene based sequence analysis results in total of 1117420 Reads across the six samples, presented among 7 phyla, 9 classes, 17 orders, 23 families and 31 genera. Genera Leptospirillum and Sulfobacillus are the main bacteria at the early and middle stages, and Leptospirillum is the main genus at the end of bioleaching. Aquabacterium and Acidovorax are special genera in sessile cells and Weissella is special in planktonic ones. Key words: pyrite dissolution; sessile cells; planktonic cells; high-throughput sequence analysis; microbial diversity; bioleaching stage Cite this article as: YIN Lu, YANG Hong-ying, LI Xiang, TONG Lin-lin, JIN Zhe-nan, ZHANG Qin. Changes of microbial diversity during pyrite bioleaching [J]. Journal of Central South University, 2020, 27(5): 1477−1483. DOI: https://doi.org/10.1007/s11771-020-4383-1.
1 Introduction Bioleaching can be widely applied in mining to win or concentrate precious metal, such as gold, from insoluble metal sulfides by biochemical oxidation [1]. During bioleaching process, microorganisms are characterized as physiologically and behaviorally integrated, highly structured microbial communities [2]. The cells undergo profound change significantly when they transfer from planktonic phase to part of the sessile phase. The researches on pyrite bioleaching can be separated into two parts: mineral part and microbial
part. For the mineral part, they focused on metal recovery [3], changes of mineral phases and the chemical compounds of the mineral surfaces [4]. Among the sulfides, pyrite has attracted much attention due to its stable structure and high iron and sulfur contents [5]. As a result of oxidation, jarosite and oxy-hydroxides can be detected in the samples [6]. YANG et al [7] found that silver ions could inhibit passivation layer formation on the chalcopyrite surface. YANG et al [8] also found that additional jarosite could inhibit passivation layer formation on chalcopyrite surface. For the m
Data Loading...
