Characteristics and diversity of endophytic bacteria in moso bamboo ( Phyllostachys eduli s) based on 16S rDNA sequencin
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ORIGINAL PAPER
Characteristics and diversity of endophytic bacteria in moso bamboo (Phyllostachys edulis) based on 16S rDNA sequencing Fang Liu1 · Zongsheng Yuan1 · Xintao Zhang1,3 · Guofang Zhang2 · Baogui Xie1
Received: 7 September 2016 / Revised: 17 February 2017 / Accepted: 5 June 2017 © Springer-Verlag GmbH Germany 2017
Abstract To understand the diversity and distribution of endophytic bacteria in moso bamboo (Phyllostachys edulis), we used 16S rDNA sequencing to investigate the characteristics and diversity of endophytic bacteria in different moso bamboo tissues. After 454 pyrosequencing, we obtained 141,269 sequences from seven moso bamboo tissue samples. The taxonomic origins of unique sequences were identified using RDP classifier. The results showed that these sequences belonged to 26 bacterial orders, including the Actinomycetales, Rickettsiales, Burkholderiales, Enterobacteriales, and Rhizobiales. Among these, Enterobacteriales was widely found in all bamboo tissues. Endophytic bacterial communities differed between the moso bamboo shoot and pole. With continuous growth and development, the number of endophytic species in the moso bamboo pole increased gradually. Keywords Phyllostachys edulis · Endophytic bacteria · 16S rDNA · 454 pyrosequencing · Diversity Communicated by Shuang-Jiang Liu. Zongsheng Yuan is the co-first author. Electronic supplementary material The online version of this article (doi:10.1007/s00203-017-1397-7) contains supplementary material, which is available to authorized users. * Guofang Zhang [email protected]; [email protected] 1
The School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
2
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
3
Nanjing Wanchen Biological Technology co., LTD, Nanjing 211299, Jiangsu, China
Introduction Moso bamboo (Phyllostachys edulis) is a member of the grass family (Gramineae) and an important forest resource in southern China; it is fast growing, easily adaptable, versatile, and of great economic value (Cui et al. 2010). The moso bamboo growing area in China, which accounts for 48% of the world’s growing area, is approximately 3.4 million hm2. Moso bamboo is widely used for structural supports in buildings and bridges, and it is also used in many aspects of daily life, such as gardening, paper making, tableware, and furniture (Qiu et al. 2001). Endophytes are microorganisms that live in the tissues and organs of plants at certain stages or all stages of their life cycles, and some endophytes can establish a symbiotic relationship with the plant (Hu et al. 2010; Liu et al. 2005). Endophytic bacteria can not only directly promote plant growth through biological nitrogen fixation (Adhikari et al. 2001) and the secretion of plant hormones, but they can also induce host plants to produce plant hormones; improve the utilization of minerals in plants; compete with pathogenic bacteria for nutrition and space; or produce antagonistic substances to directly
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