Physiological and transcriptomic analyses of brassinosteroid function in moso bamboo ( Phyllostachys edulis ) seedlings
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ORIGINAL ARTICLE
Physiological and transcriptomic analyses of brassinosteroid function in moso bamboo (Phyllostachys edulis) seedlings Zhe Zhang1 · Xuelian Yang1,3 · Ling Cheng1,2 · Zejun Guo1 · Huiyuan Wang1 · Weihuang Wu1 · Kihye Shin1 · Jinyao Zhu1 · Xiaoli Zheng1 · Jianghu Bian1,2 · Yangchen Li1,2 · Lianfeng Gu1 · Qiang Zhu1 · Zhi‑Yong Wang3 · Wenfei Wang1,2 Received: 22 February 2020 / Accepted: 17 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion This study demonstrates that brassinosteroid is essential for seedling and shoot growth in moso bamboo. Abstract The shoot of moso bamboo is known to grow extremely fast. The roles of phytohormones in such fast growth of bamboo shoot remain unclear. Here we reported that endogenous brassinosteroid (BR) is a major factor promoting bamboo shoot internode elongation. Reducing endogenous brassinosteroid level by its biosynthesis inhibitor propiconazole stunted shoot growth in seedling stage, whereas exogenous BR application promoted scale leaf elongation and the inclination of lamina joint of leaves and scale leaves. Genome-wide transcriptome analysis identified hundreds of genes whose expression levels are altered by BR and propiconazole in shoots and roots of bamboo seedling. The data show that BR regulates cell wall-related genes, hydrogen peroxide catabolic genes, and auxin-related genes. Our study demonstrates an essential role of BR in fast growth bamboo shoots and identifies a large number of BR-responsive genes in bamboo seedlings. Keywords Brassinosteroid · Fast growing · Moso bamboo · Phytohormones · Propiconazole · Scale leaf Abbreviations BR Brassinosteroid BL Brassinolide DEG Differentially expressed gene eBL 2,4-Epicastasterone Communicated by Dorothea Bartels. Zhe Zhang and Xuelian Yang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03432-z) contains supplementary material, which is available to authorized users. * Wenfei Wang [email protected] 1
Basic Forestry and Proteomics Research Center, Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China
2
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China
3
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA
GO Gene ontology PPZ Propiconazole ROS Reactive oxygen species
Introduction Bamboo is one of the most important non-timber forest plants in the world, which has considerable economic, ecological and cultural value. Bamboo is the woody member of the grass family, Poaceae. Moso bamboo, Phyllostachys edulis, is the largest specie in total bamboo growth area of Asia (Fu 2001). Moso bamboo is known for its fast-growing shoot, which can grow as fast as 1 m per 24 h. Bamboo shoot growth is attributed to sequential internode elongation, which is caused by cell division in the intercalary meristem and subsequent cell elongation in the following elongation zon
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