Characterization and resuscitation of viable but nonculturable Vibrio alginolyticus VIB283

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ORIGINAL PAPER

Characterization and resuscitation of viable but nonculturable Vibrio alginolyticus VIB283 Meng Du · Jixiang Chen · Xiaohua Zhang · Aijuan Li · Yun Li

Received: 14 February 2007 / Revised: 28 March 2007 / Accepted: 2 April 2007 / Published online: 10 May 2007 © Springer-Verlag 2007

Abstract The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4°C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to undetectable levels (