Characterization, inheritance, and molecular study of opaque leaf mutant in mungbean ( Vigna radiata (L.) Wilczek)
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J. Crop Sci. Biotech. 2010 (December) 13 (4) : 219 ~ 226 DOI No. 10.1007/s12892-010-0059-y RESEARCH ARTICLE
Characterization, Inheritance, and Molecular Study of Opaque Leaf Mutant in Mungbean (Vigna radiata (L.) Wilczek) Ornuma Rungnoi1,2 , Sonthichai Chanprem1,3, Theerayut Toojinda4, Ian Godwin5, Chris Lambrides5, Peerasak Srinives1,3* Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand Department of Plant Production Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand 3 Department of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand 4 Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand 5 School of Land and Food Science, University of Queensland, St Lucia, Brisbane, Queensland 4067, Australia 1
2
Received: May 22, 2010 / Revised: October 26, 2010 / Accepted: November 25, 2010 Ⓒ Korean Society of Crop Science and Springer 2010
Abstract Chlorophyll-deficiency mutants are useful as genetic markers and as materials for studying the photosynthesis process. We characterized the inheritance of the gene controlling an opaque leaf (OL) trait in mungbean (Vigna radiata (L.) Wilczek). An F2 population was developed from the cross between the OL mutant and 'Berken', an Australian mungbean cultivar, to study inheritance and molecular tagging. The population was advanced by selfing to produce F5 lines from which two normal lines and two OL lines were randomly chosen to study chlorophyll content, seed growth and development, and seed cell morphology. The chlorophyll content in opaque leaf was lower than normal and thus was expected to have lower photosynthetic activity. This resulted in yellowish and shrinking pods and seeds within 15 days after flowering, while those from normal plants extended their growth up to 18 days. The cotyledon transfer cells of the OL plants deformed at 12 days and deteriorated at 14 days after flowering. The OL trait was controlled by a single recessive ol gene which was independent from the genes controlling petiole color and growth habit. We used 193 AFLP primer combinations to tag this gene and found that the marker AGG/ATA was linked with the ol gene at a distance of 3.4 cM. Key words: Characterization, inheritance, molecular marker, mungbean, opaque leaf, Vigna radiata
Introduction Induced mutation in mungbean (Vigna radiata (L.) Wilczek), a self-pollinated field crop, can result in mutation relating to yield attributes, pest resistance, and higher nodulation (Gupta 1996). Alterations in pigmentation, especially chlorophyll pigments, are the most common induced mutations found in crop plants (Nelson 1967). Deficiency in chlorophyll content can lead to abnormal leaf coloration (Nair and Tomar 2001). In mungbean, chlorophyll mutations can be classified into albina, xantha, viridis, maculate, chlorina, tigrina, lutercent, striata, virescent,
Peerasak Srinives ( ) Email: agrpss@y
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