Molecular characterization of Tomato leaf curl virus infecting hollyhock ( Alcea rosea L. ) in India
- PDF / 4,106,785 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 90 Downloads / 271 Views
RESEARCH ARTICLE
Molecular characterization of Tomato leaf curl virus infecting hollyhock (Alcea rosea L. ) in India K. V. Ashwathappappa1 · V. Venkataravanappa1,2 · C. N. Lakshminarayana Reddy3 · M. Krishna Reddy1 Received: 16 April 2019 / Revised: 3 February 2020 / Accepted: 16 March 2020 © Indian Phytopathological Society 2020
Abstract The hollyhock plants exhibiting typical symptoms produced by begomovirus were collected and subjected to PCR analysis using specific primers to amplify viral DNA. PCR amplification and whole genome sequencing indicated the involvement of a DNA virus in hollyhock infection. The full-length genome of the virus infecting hollyhock has shown maximum nucleotide (nt) identity of 91% with Tomato leaf curl virus (ToLCV) (KP178730 and AY754812) infecting tomato in India. As per the presently applicable classification for DNA viruses (91% nt identity), the virus infecting hollyhock is a strain of ToLCV. The betasatellite associated with hollyhock leaf curl disease showed maximum nt identity of 88.4–88.9% with Cotton leaf curl Bangalore betasatellite (CLCuBaB) infecting cotton and okra in south India. Further recombination analysis showed that the genome fragments of the virus infecting hollyhock has been derived from already prevailing begomoviruses infecting diverse crops resulting in the evolution of recombinant virus. This is the first report of ToLCV infection in Hollyhock. Keywords Tomato leaf curl virus (ToLCV) · Cotton leaf curl Bangalore betasatellite (CLCuBaB) · Polymerase chain reaction · Phylogenetic analysis
Introduction The plant-infecting viruses which belong to the family Geminiviridae is comprised of ssDNA genome, encapsidated in twinned quasi-icosahedral particles (size of 2.5–3.1 kb) and are wide spread in most parts of the world (Stanley 1985). Based on host range, genome organization and insect Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42360-020-00221-2) contains supplementary material, which is available to authorized users. * V. Venkataravanappa [email protected] * C. N. Lakshminarayana Reddy [email protected] 1
ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore, Karnataka 560089, India
2
Division of Plant Pathology, Central Horticultural Experiment Station, ICAR-Indian Institute of Horticultural Research, Chettalli, Madikeri (District), Bangalore, Karnataka 57124, India
3
Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore, Karnataka 560065, India
vectors, these are classified into nine genera (Becurtovirus, Begomovirus, Capulavirus, Curtovirus, Eragrovirus, Grablovirus, Mastrevirus, Topocuvirus and Turncurtovirus) (Zerbini et al. 2017). Among these, begomoviruses are the most important viruses infecting different crops worldwide and are transmitted by different biotypes of whitefly Bemisia tabaci in a persistent circulative and non-propagative manner. The genome of these viruses are monopartite (sin
Data Loading...
