Characterization of a new subgroup of Rhizoctonia solani anastomosis group 3 (AG-3 TM) associated with tomato leaf bligh
- PDF / 1,027,044 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 86 Downloads / 199 Views
FUNGAL DISEASES
Characterization of a new subgroup of Rhizoctonia solani anastomosis group 3 (AG‑3 TM) associated with tomato leaf blight Tomoo Misawa1,6 · Daisuke Kurose2 · Kuniaki Shishido3 · Takeshi Toda4 · Shiro Kuninaga5 Received: 23 February 2020 / Accepted: 30 March 2020 © The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2020
Abstract Ten Rhizoctonia solani AG-3 isolates obtained from blighted tomato leaves [nine isolates, collected from three regions of Japan (Akita, Okayama, and Fukushima) and the United States] and from soil (one isolate, collected from Australia) were examined for sequence variations in the rDNA-ITS, rDNA-IGS1 regions, and tef-1α gene. Three loci of 16 AG-3 PT (potato type) and eight AG-3 TB (tobacco type) isolates were also sequenced to evaluate genetic relationships with these 10 isolates. In the sequence comparisons of these loci, all 10 AG-3 isolates shared high sequence similarity (99.4–100% for rDNA-ITS, 99.1–100% for rDNA-IGS1, and 98.2–100% for tef-1α) to each other, but a comparatively low similarity (95.4–98.8% for rDNA-ITS, 94.7–98.3% for rDNA-IGS1, and 93.5–97.2% for tef-1α) to AG-3 PT and AG-3 TB isolates. The 10 AG-3 isolates were genetically closer to AG-3 PT than to AG-3 TB on the basis of rDNA-ITS and tef-1α. A phylogenetic tree based on these three loci indicated that the 10 isolates form a new clade separated from AG-3 PT and TB populations. These results indicate that the 10 isolates from tomato and soil represent a new subgroup distinct from AG-3 PT and TB. New subgroup AG-3 TM (tomato type) is proposed. This study is the first report on the phylogenetic analysis of R. solani isolates based on the combined data from the rDNA-ITS, rDNA-IGS1 regions, and tef-1α gene. Keywords Phylogetetics · Potato · Rhizoctonia solani AG-3 · Tobacco · Tomato
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10327-020-00943-1) contains supplementary material, which is available to authorized users. * Tomoo Misawa misawa‑[email protected] 1
Donan Agricultural Experiment Station, Hokkaido Research Organization, Hokuto, Hokkaido 041‑1201, Japan
2
CABI Europe-UK, Bakeham Lane, Egham TW20 9TY, Surrey, UK
3
Agriculture, Forestry and Fisheries Department, Fukushima Prefectural Government, Sugitsumacho, Fukushima 960‑8670, Japan
4
Faculty of Bioresource Science, Akita Prefectural University, Shimo‑shinjo, Akita 010‑0195, Japan
5
Health Sciences University of Hokkaido, Ishikari‑Tobetsu, Hokkaido 061‑0293, Japan
6
Present Address: Corporate Department, Hokkaido Research Organization, Sapporo, Hokkaido 060‑0819, Japan
The Rhizoctonia solani species complex comprises pathogens that are destructive to a wide range of host plant species. Isolates of R. solani are divided into anastomosis groups (AGs) 1–13 (Carling et al. 2002). R. solani AG-3 isolates have long been known to cause black scurf of potato, but additional AG-3 isolates have been found that cause Rhizoctonia lea
Data Loading...
