Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani
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ANNOTATED SEQUENCE RECORD
Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani Siwei Li1 · Yuting Li1 · Chenghui Hu1 · Chenggui Han1 · Tao Zhou1 · Can Zhao1,2 · Xuehong Wu1 Received: 9 March 2020 / Accepted: 5 April 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A double-stranded RNA (dsRNA) segment was identified in Rhizoctonia solani anastomosis group (AG)-2-2IIIB, the primary causal agent of Rhizoctonia crown and root rot of sugar beet. The dsRNA segment represented the genome replication intermediate of a new mitovirus that was tentatively designated as "Rhizoctonia solani mitovirus 39" (RsMV-39). The complete sequence of the dsRNA was 2805 bp in length with 61.9% A+U content. Using either the fungal mitochondrial or universal genetic code, a protein of 840 amino acids containing an RNA-dependent RNA polymerase (RdRp) domain was predicted with a molecular mass of 94.46 kDa. BLASTp analysis revealed that the RdRp domain of RsMV-39 had 43.55% to 72.96% sequence identity to viruses in the genus Mitovirus, and was the most similar (72.96% identical) to that of Ceratobasidium mitovirus A (CbMV-A). Phylogenetic analysis based on RdRp domains clearly showed that RsMV-39 is a member of a distinct species in the genus Mitovirus of the family Mitoviridae. This is the first full genome sequence of a mycovirus associated with R. solani AG-2-2IIIB. Sugar beet is widely grown throughout the world and represents the second-largest sugar crop [1]. Rhizoctonia crown and root rot of sugar beet leads to yield losses of 50% or more, and Rhizoctonia solani AG-2-2IIIB is the predominant causal agent of this disease [2, 3]. Mycoviruses are widespread in all major groups of plant pathogenic fungi, and because they are sometimes associated with hypovirulence, mycoviruses could be potentially utilized as biological control agents to combat plant diseases caused by fungi [4]. Although the majority of known mycoviruses with dsRNA genomes are packaged in isometric particles, an increasing number of unencapsidated Handling Editor: Ioly Kotta-Loizou. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04664-w) contains supplementary material, which is available to authorized users. * Can Zhao [email protected] * Xuehong Wu [email protected] 1
College of Plant Protection, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China
College of Horticulture, China Agricultural University, Haidian District, Beijing 100193, People’s Republic of China
2
mycoviruses with positive single-stranded RNA (+ssRNA) genomes has been reported [5]. Up to now, +ssRNA mycoviruses have been classified into eight families, including Alphaflexiviridae, Gammaflexiviridae, Hypoviridae, Narnaviridae, Barnaviridae, Endornaviridae, Botourmiaviridae, and the proposed family "Fusariviridae" [6–9]. Currently, the mycoviruses isolated and characterized from R. solani mainly belong to the families Narn
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