Characterization of articular chondrocytes isolated from 211 osteoarthritic patients
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ORIGINAL PAPER
Characterization of articular chondrocytes isolated from 211 osteoarthritic patients Matteo Lagana` • Chiara Arrigoni • Silvia Lopa • Valerio Sansone • Luigi Zagra • Matteo Moretti • Manuela Teresa Raimondi
Received: 11 December 2012 / Accepted: 22 March 2013 / Published online: 3 April 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract We analyzed specific features of chondrocytes as cellular yield, cell doubling rates and the dependence between these parameters and patientrelated data in a set of 211 osteoarthritic (OA) patients undergoing total joint replacement. For each patient the data available were joint type, age and gender. Knee samples chosen randomly among all biopsies were graded according to ICRS score. Patients’ age ranged between 30 and 90 years with a mean age of 66 ± 9.7 years. Patients were divided into age classes and statistically significant differences in proliferation rate at passage 1 were found between chondrocytes derived from young and old donors, with the last ones characterized by a lower proliferation rate. A similar
Chiara Arrigoni andSilvia Lopa contributed equally to this work. Matteo Moretti and Manuela Teresa Raimondi contributed equally to this work. M. Lagana` M. T. Raimondi (&) LaBS, Department of Structural Engineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy e-mail: [email protected] C. Arrigoni S. Lopa Cell and Tissue Engineering Laboratory, Gruppo Ospedaliero San Donato Foundation, Milan, Italy
trend was observed for proliferation rate at passage 2. For all the samples, cellular yields ranged between 0.1 and 5.5 million cells/g of tissue. No significant correlation was observed between the level of cartilage degeneration (ICRS score) and cellular yield and proliferation rates. However, in samples with a high degree of cartilage degeneration (ICRS score 4) the cellular yield was lower compared to the other three groups (ICRS scores 1–3). In this study we performed a systematic characterization of basic parameters of chondrocytes originating from a wide group of OA patients. Considering the use of autologous chondrocytes in chondral treatments, the characterization of cell basic features may represent an important step to determine the quality of the cell source which is a major determinant in the outcome of cell-based therapies. Keywords Osteoarthritis Articular chondrocyte Age Yield ICRS score L. Zagra Hip Department, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy M. Moretti Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy
V. Sansone Orthopaedic Department, IRCCS Galeazzi Orthopaedic Institute, University of Milan, Milan, Italy
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Introduction Osteoarthritis (OA) is a highly disabling pathology which is worldwide investigated by the scientific community due to its increasing diffusion. This agedependent degenerative disease induces the progressive damage of articular cartilage and subchondral bone and can eventually lead to the com
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