Characterization of microsatellite markers isolated from members of Oreocarya (Boraginaceae)
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MICROSATELLITE LETTERS
Characterization of microsatellite markers isolated from members of Oreocarya (Boraginaceae) Gerald E. Bresowar • Mitchell E. McGlaughlin
Received: 9 September 2013 / Accepted: 23 September 2013 / Published online: 2 October 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Genus Oreocarya (Boraginaceae) contains approximately 62 species, many rare or uncommon. The genus is endemic to western North America with a center of diversity in the Colorado Plateau and Great Basin of the U.S. Rarer members are often restricted to regionally uncommon soil types. Twelve polymorphic microsatellite loci were isolated from three taxa, Oreocarya bakeri, O. revealii, and O. paradoxa, and were screened for variability in 56 individuals of O. revealii and O. paradoxa. Cross amplification was assessed using 7 additional taxa. Diversity was observed to be high, with mean numbers of alleles per locus ranging from 4.0 to 15.5. Mean expected and observed heterozygosities ranged from 0.32 to 0.87 and 0.16 to 0.70, respectively. With its frequent development of soil restricted lineages, Oreocarya is an interesting system for investigations of edaphically associated rarity. The development of these new loci will aid evolutionary and conservation investigations in this system. Keywords Colorado Plateau Oreocarya Cryptantha Microsatellite
The genus Oreocarya Payson (formerly subgenus Oreocarya, genus Cryptantha Lehmann ex. G.Don; family Boraginaceae, subfamily Boraginoideae) contains approximately 62 species and 71 taxa. Oreocarya is endemic to Electronic supplementary material The online version of this article (doi:10.1007/s12686-013-0056-9) contains supplementary material, which is available to authorized users. G. E. Bresowar (&) M. E. McGlaughlin School of Biological Sciences, University of Northern Colorado, 501 20th Ave., Greeley, CO 80639, USA e-mail: [email protected]
western North America with its center of species diversity in the Colorado Plateau and Great Basin of the western United States. The genus consists of xeric adapted, semi woody and relatively slow growing plants, with several halophytic members. Species distributions vary from widespread and relatively common, to narrowly endemic and restricted to uncommon soil types (Cronquist et al. 1984). One rare taxon, O. revealii, is restricted to gypsum soils of the Paradox Formation in eastern Colorado, and is considered a sensitive species by the Bureau of Land Management. O. revealii has a global rarity rank of G1G2, indicating that it is critically imperiled due to low population occurrence and/or highly restricted range requirements. Here we report the characterization of twelve new variable loci isolated from three taxa within Oreocarya; O. bakeri Greene, O. paradoxa A. Nelson, and O. revealii W.A. Weber and R.C. Whittmann. Genomic DNA was isolated from leaf tissue using a modified CTAB extraction protocol (Friar 2005). Microsatellite libraries were constructed individually for three taxa, O. bakeri, O. paradoxa, and O.
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