Characterization of high- and low-molecular-weight glutenin subunits from Chinese Xinjiang wheat landraces and historica
- PDF / 647,160 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 49 Downloads / 179 Views
ORIGINAL ARTICLE
Characterization of high- and low-molecular-weight glutenin subunits from Chinese Xinjiang wheat landraces and historical varieties Shoufen Dai1 • Dongyang Xu1 • Yongliang Yan2 • Zhaojin Wen1 • Jinbo Zhang2 Haixia Chen1 • Zifeng Lu2 • Haoyuan Li1 • Hua Cong2 • Yuming Wei1 • Youliang Zheng1 • Zehong Yan1
•
Revised: 10 April 2019 / Accepted: 3 April 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract Landraces and historical varieties are necessary germplasms for genetic improvement of modern cereals. Allelic variations at the Glu-1 and Glu-3 loci in 300 common wheat landraces and 43 historical varieties from Xinjiang, China, were evaluated by Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and allele-specific molecular markers. Among the materials investigated, three, nine, and seven alleles were identified from the Glu-A1, Glu-B1, and Glu-D1 loci, respectively, and a total of 26 high-molecular-weight glutenin subunit (HMW-GS) combinations were found, of which 18 combinations were identified in landraces and historical varieties. Allelic frequency of HMW-GS combinations null, 7 ? 8, 2 ? 12 was found to be the highest in both the landraces (63.3%) and historical varieties (39.5%). Besides, some distinctive HMW-GS alleles, such as the novel Glu-B1 allele 6.1* ? 8.1* and Glu-D1 alleles 2.6 ? 12, 2.1 ? 10.1, and 5** ? 10 were observed in Xinjiang wheat landraces. Among the Glu-A3 and Glu-B3 loci of landraces and historical varieties, a total of eight and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13197-020-04414-5) contains supplementary material, which is available to authorized users. & Hua Cong [email protected] & Zehong Yan [email protected] 1
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People’s Republic of China
2
Research Institute of Crop Germplasm Resource, Xinjiang Academy of Agricultural Science, Urumqi 830091, Xinjiang-Uygur Autonomous District, People’s Republic of China
nine alleles were found, respectively. At each locus, two novel alleles were identified. A total of 33 low-molecularweight glutenin subunit (LMW-GS) combinations of GluA3 and Glu-B3 were identified, with 31 and 14 combinations occurring in landraces and historical varieties, respectively, but only 10 combinations shared by both of them. As Glu-D1, Glu-A3, and Glu-B3 have highest contribution to the end-use quality and processing properties as compared to Glu-A1, Glu-B1, and Glu-D3 locus, the novel or distinctive HMW-GS and LMW-GS alleles in these loci could potentially be utilized for the improvement in the quality of modern wheat. Keywords Glu-1, Glu-3 SDS-PAGE, Allele specific PCR marker, Genetic diversity Abbreviations HMW-GS High-molecular-weight glutenin subunits LMW-GS Low-molecular-weight glutenin subunits SDS-PAGE Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis STS Sequence tagged site PCR Polymerase chain reaction H The genetic dispersion index LA
Data Loading...
