Characterization of left ventricular myocardial sodium-glucose cotransporter 1 expression in patients with end-stage hea
- PDF / 3,865,365 Bytes
- 16 Pages / 595.276 x 790.866 pts Page_size
- 25 Downloads / 186 Views
ORIGINAL INVESTIGATION
Cardiovascular Diabetology Open Access
Characterization of left ventricular myocardial sodium‑glucose cotransporter 1 expression in patients with end‑stage heart failure Alex Ali Sayour1* , Attila Oláh1, Mihály Ruppert1, Bálint András Barta1, Eszter Mária Horváth2, Kálmán Benke1, Miklós Pólos1, István Hartyánszky1, Béla Merkely1 and Tamás Radovits1
Abstract Background: Whereas selective sodium-glucose cotransporter 2 (SGLT2) inhibitors consistently showed cardiovascular protective effects in large outcome trials independent of the presence of type 2 diabetes mellitus (T2DM), the cardiovascular effects of dual SGLT1/2 inhibitors remain to be elucidated. Despite its clinical relevance, data are scarce regarding left ventricular (LV) SGLT1 expression in distinct heart failure (HF) pathologies. We aimed to characterize LV SGLT1 expression in human patients with end-stage HF, in context of the other two major glucose transporters: GLUT1 and GLUT4. Methods: Control LV samples (Control, n = 9) were harvested from patients with preserved LV systolic function who went through mitral valve replacement. LV samples from HF patients undergoing heart transplantation (n = 71) were obtained according to the following etiological subgroups: hypertrophic cardiomyopathy (HCM, n = 7); idiopathic dilated cardiomyopathy (DCM, n = 12); ischemic heart disease without T2DM (IHD, n = 14), IHD with T2DM (IHD + T2DM, n = 11); and HF patients with cardiac resynchronization therapy (DCM:CRT, n = 9, IHD:CRT, n = 9 and IHD-T2DM:CRT, n = 9). We measured LV SGLT1, GLUT1 and GLUT4 gene expressions with qRT-PCR. The protein expression of SGLT1, and activating phosphorylation of AMP-activated protein kinase (AMPKα) and extracellular signalregulated kinase 1/2 (ERK1/2) were quantified by western blotting. Immunohistochemical staining of SGLT1 was performed. Results: Compared with controls, LV SGLT1 mRNA and protein expressions were significantly and comparably upregulated in HF patients with DCM, IHD and IHD + T2DM (all P
Data Loading...
