Characterization of Ogura CMS fertility-restored interspecific hybrids and backcross progenies from crosses between broc
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Characterization of Ogura CMS fertility-restored interspecific hybrids and backcross progenies from crosses between broccoli and rapeseed Chun-qing Liu Zhu-jie Xie
. Guang-qing Li . Xue-qin Yao . Lei Huang . Xin-yan Wu .
Received: 15 June 2020 / Accepted: 25 October 2020 Ó Springer Nature B.V. 2020
Abstract Interspecific F1 hybrids were obtained from crosses between two Ogura cytoplasmic male sterility (Ogura CMS) broccoli lines (B14 and 137) and a rapeseed restorer line to introgress the Ogura CMS’s fertility-restoration gene Rfo from rapeseed into broccoli. Ten Rfo-positive interspecific, triploid plant progenies, phenotypically between rapeseed and broccoli, were obtained but still contained multiple rapeseed genes, with reduced fertility and seed setting potential under natural pollination. For fertility and seed setting improvement, successive backcrosses with broccoli were carried out to yield BC2 progenies. Screening revealed six plants with Rfo among 25 BC2
Chun-qing Liu and Guang-qing Li have contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10681-020-02721-8) contains supplementary material, which is available to authorized users. C. Liu G. Li X. Yao L. Huang Z. Xie (&) Institute of Horticulture, Shanghai Academy of Agricultural Sciences, National Center for Vegetable Improvement (Shanghai Branch), Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China e-mail: [email protected] X. Wu School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China
progenies, which were investigated for agronomic traits, ploidy determination, pollen viability and SSR (simple sequence repeat) markers to evaluate for ideal individuals closer to broccoli. Pollen viability tests were carried out at different stages for various hybrid parents. The results were as follows: 137-F1 (68.54%) [ B14-F1 (65.32%) [ 137-BC1 (48.15%) [ B14-BC1 (32.41%). Although there were significant differences among the BC1 and BC2 individuals, the genetic background of BC2 was closer to the parent broccoli compared with that of BC1 plants. The existence of the Rfo gene in broccoli not only facilitates broccoli germplasm innovation, combination of interspecific hybridization and backcrossing, but may also shed light on trait segregation in different generations. Keywords Interspecific hybridization Rfo gene Brassica oleracea var. italic Fertility-restored
Introduction Broccoli (Brassica oleracea var. italica) is native to the Mediterranean region, and constitutes an important vegetable that provides an excellent source of many health-promoting phytochemicals such as vitamins K and C, chromium, folic acid and especially glucosinolates, which are under scientific investigation for their role in cancer prevention (Vasanthi et al. 2009). It
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is well established that broccoli exhibits high heterosis, and commercial hybr
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