Embryo rescue response and genetic analyses in interspecific crosses of oilseed Brassica species
- PDF / 1,015,258 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 79 Downloads / 260 Views
PLANT TISSUE CULTURE
Embryo rescue response and genetic analyses in interspecific crosses of oilseed Brassica species Romana Sharmin Ripa 1 & Mohammad Rashid Arif 1 & M. Thoihidul Islam 1 & Arif Hasan Khan Robin 1 Received: 19 February 2020 / Accepted: 17 August 2020 / Editor: Yong Eui Choi # The Society for In Vitro Biology 2020
Abstract Embryo rescue technique is widely utilized to save developing embryo from abortion in interspecific hybridization of plants. In Brassica species, success of an embryo rescue depends on the age of embryo, developmental stage of embryo, and media composition. In this study, ten crosses were conducted between diploid oilseed species B. rapa × tetraploid B. napus and B. rapa × tetraploid B. juncea along with their reciprocal crosses. The immature embryos from parents and crosses were cultured at 14 d after pollination in MS and ½ MS medium without any growth regulators for direct embryogenesis. Embryos were found at different stages of development during isolation, from globular to cotyledonary, indicating that rate of embryo development depends on genotype. Embryos isolated at the cotyledonary stage showed the highest regeneration percentage (av. 83.29%) and required the lowest time to regenerate (5.8 d) compared with another type of embryo, torpedo stage, cultured. Embryo rescued in ½ MS media accounted for 10–51%, 4.0–61%, 6.0–21%, and 5.0–43% greater shoot length, root length, number of leaves, and days to flowering, respectively, in different cross combinations compared with full-strength MS media. The results indicated that the rate of regeneration from embryo is higher in ½ MS media compared with full-strength MS media. Percent regeneration from the cultured embryos differed significantly in different cross combinations. Days required for regeneration from embryos, length of siliqua, and the number of ovules per siliqua accounted for high heterosis and broad sense heritability. The findings of this experiment would be useful in developing cost-effective embryo rescue protocol and desired interspecific hybrids through embryo rescue in Brassica species. Keywords Oilseed Brassica . Interspecific hybridization . Embryo rescue . MS and ½ MS media . Heterosis and heritability
Introduction The oleiferous rape and mustard under the Brassicaceae family are among the worlds’ top three oilseed producing crop (Katche et al. 2019). The gross oilseed production recorded 75 million tons in the year of 2018, globally (http://www.fao.org/
* Arif Hasan Khan Robin [email protected] Romana Sharmin Ripa [email protected] Mohammad Rashid Arif [email protected] M. Thoihidul Islam [email protected] 1
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
faostat/en/#data/QC/visualize). In Bangladesh, rapeseed mustard has an increasing demand of mustard, but Bangladesh can fulfill only one-fifth of her domestic demand (BBS 2017; https://www.mordorintelligence.com/industryreports/bangladesh-mustard-oil-market). Considering the globa
Data Loading...
