Characterization of the complete chloroplast genome sequence of Lysionotus pauciflorus (Gesneriaceae)
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TECHNICAL NOTE
Characterization of the complete chloroplast genome sequence of Lysionotus pauciflorus (Gesneriaceae) Ting Ren1 · Wei Zheng2 · Kai Han1 · Shuyun Zeng1 · Jianhua Zhao1 · Zhan-Lin Liu1
Received: 3 October 2016 / Accepted: 23 October 2016 © Springer Science+Business Media Dordrecht 2016
Abstract Lysionotus pauciflorus, an evergreen subshrub with high medicinal and ecological values, mainly distributed in subtropical montane regions of China. In this study, we reported its complete chloroplast (cp) genome from Next Generation Sequencing data. The whole genome was 153,856 bp in length, containing a pair of inverted repeat region (IRs) of 25,465 bp, a large single copy (LSC) region of 85,087 bp and a small single copy (SSC) region of 17,839. The genome contained 133 genes, including 88 protein-coding genes (80 PCG species), 37 tRNA genes (28 tRNA species) and 8 rRNA genes (4 rRNA species). Among these genes, 12 genes harbored a single intron and 3 genes harbored 2 introns. The majority of gene species occurred as a single copy and one tRNA gene occurred as three copies. The overall AT content of L. pauciflorus cp genome is 62.5%. A maximum parsimony (MP) phylogenetic analysis demonstrated a close relationship between L. pauciflorus and Boea hygrometrica. Keywords Lysionotus pauciflorus · Chloroplast genome · Illumina sequencing · Phylogenetic analysis
Lysionotus pauciflorus, an evergreen subshrub mainly growing as scattered individuals in montane regions of South China, is of high medicinal value and has been
* Zhan-Lin Liu [email protected] 1
Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Northwest University, Xi’an 710069, Shaanxi, China
2
Xi’an Third Hospital, Xi’an 710082, Shaanxi, China
widely used to treat traumatic injury, tuberculosis and rheumatic pains in Miao regions (Wang et al. 2009). For its strong drought resistance and adaptability to Rocky Desertification Area, L. pauciflorus has become an important plant for ecological management and quarries reforestation (Liu et al. 2014). But due to overexploitation of the natural resources and habitat fragmentation, population size of L. pauciflorus has dramatically decreased (Wang et al. 2009). Measures for conservation and restoration of this species are urgently needed. An improved understanding of its genomics information would be definitely fundamental to formulate the comprehensive conservation strategy. Here, we presented the complete chloroplast genome of L. pauciflorus (GenBank: KX752081) based on the Illumina pairend sequencing data. An individual of L. pauciflorus was sampled from Hualong Mountain (Shaanxi, China; 109.36°E, 32.03°N). Total genomic DNA was extracted from silicon-dried leaves with the modified CTAB method (Doyle 1987). DNA sample and voucher specimen were deposited in the Evolutionary Botany Laboratory (EBL), Northwest University. The whole-genome sequencing was performed with the 150 bp pair-end sequencing method by the Illumina Hiseq 2500 Pl
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