Characterization of transcription activation domain of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulen
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ORIGINAL ARTICLE
Characterization of transcription activation domain of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis Ziyang Zhang1,2 · Ling Cheng3 · Weihua Zhang4 · Jianlin Hu1,2 · Yan Liu1,2 · Yuanzhen Lin1,2 Received: 23 May 2019 / Accepted: 26 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract SUMOylation in plants is associated with biotic and abiotic stress responses, flowering, and other aspects of development. ICE1 protein SUMOylation by the SUMO E3 ligase SIZ1 plays important roles in plant cold tolerance. Here, we reported the subcellular localization of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis. The genes EcaICE1 and EcaSIZ1 were isolated by homologous cloning. The subcellular localization analysis showed that EcaICE1 was located in nucleus. Bimolecular fluorescence complementation (BiFC) analysis revealed that EcaICE1 could interact with EcaSIZ1 in the nucleus of Nicotiana benthamiana leaves. Moreover, yeast two-hybrid assay confirmed that the amino acid region from position 84 to 126 in EcaICE1 was critical for the strong transactivation activity of EcaICE1 and that the C-terminal region from position 361 to 557 in EcaICE1 was the key region for its interaction with EcaSIZ1 using different truncated lengths of non-transactivation activity of EcaICE1 as the bait protein. Collectively, our results showed that EcaICE1 may have a SUMOylation pathway similar to Arabidopsis thaliana. Keywords Eucalyptus camaldulensis · EcaICE1 · EcaSIZ1 · Protein interaction · SUMOylation
Introduction Low temperature is an environmental factor that can restrict the growth, development, geographical distribution, and production of plants (Janmohammadi et al. 2015). To overcome Communicated by Peterson. Ziyang Zhang, Ling Cheng and Weihua Zhang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00468-020-01994-9) contains supplementary material, which is available to authorized users. * Yuanzhen Lin [email protected] 1
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China
3
Guangzhou Genedenovo Biotechnology Company Limited, Guangzhou 510642, China
4
Guangdong Academy of Forestry, Guangzhou 510640, China
low-temperature stress, a series of complex plant response mechanisms can be activated at physiological and molecular levels (Shi et al. 2018). Research reveals that a large number of cold-regulated genes (CORs) are induced after plant cold acclimation. These CORs genes usually contain DRE/CRT cis-acting elements and can interact with transcription factors of the CBF families (Stockinger et al. 1997). CBFs are key transcription factors of low-temperature signaling pathways and play important roles in enhancing plant cold tolerance (Chinnusamy et al. 2007). CBFs are ind
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