Characterization of two newly isolated Staphylococcus aureus bacteriophages from Japan belonging to the genus Silviaviru
- PDF / 967,428 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 101 Downloads / 191 Views
BRIEF REPORT
Characterization of two newly isolated Staphylococcus aureus bacteriophages from Japan belonging to the genus Silviavirus Naoya Kitamura1 · Eri Sasabe1 · Shigenobu Matsuzaki2,3 · Masanori Daibata2 · Tetsuya Yamamoto1 Received: 6 January 2020 / Accepted: 24 June 2020 / Published online: 3 August 2020 © The Author(s) 2020
Abstract Two Staphylococcus aureus bacteriophages, KSAP7 and KSAP11, were isolated from sewage and characterized. Based on morphology and DNA sequences, they were assigned to the genus Silviavirus, subfamily Twortvirinae, family Herelleviridae, whose members are hypothesized to be suitable for bacteriophage therapy. The KSAP7 and KSAP11 genomes were 137,950 and 138,307 bp in size, respectively. Although their DNA sequences were almost identical, evidence of site-specific DNA rearrangements was found in two regions. Changes in the number of PIEPEK amino acid sequence repeats encoded by orf10 and the insertion/deletion of a 541-bp sequence that includes a possible tail-related gene were identified. Increased resistance of pathogenic bacteria to antibacterial agents has prompted the development of alternatives and/or supplements to current antibacterial therapies. One of the most potent antibiotic-independent alternatives is bacteriophage (phage) therapy [1]. In phage therapy, phages (viruses specific for bacteria) or phage products such as endolysin and depolymerase [2–4] function as antibacterial agents. A large number of phages have been isolated and characterized for the purpose of developing phage therapies. Virulent phages, which do not exhibit a lysogenic cycle, are generally considered more suitable than lysogenic phages due to the low likelihood of superinfection exclusion, in which lysogens act against the same type of phage, and of the transfer of pathogenic genes [5]. Handling Editor: Johannes Wittmann. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04749-6) contains supplementary material, which is available to authorized users. * Naoya Kitamura nkitamura@kochi‑u.ac.jp 1
Department of Oral and Maxillofacial Surgery, Kochi Medical School, Kochi University, Nankoku, Kochi 783‑8505, Japan
2
Department of Microbiology and Infection, Kochi Medical School, Kochi University, Nankoku, Kochi 783‑8505, Japan
3
Present Address: Department of Medical Laboratory Science, Faculty of Health Sciences, Kochi Gakuen University, Kochi, Kochi 780‑0955, Japan
Most of the therapeutic candidate phages reported to date have been assigned to the order Caudovirales (namely tailed phages) [6, 7]. Phages with a tail and contractile sheath, which have been traditionally assigned to the family Myoviridae, have now been reorganized into three families based on DNA sequence: Myoviridae, Herelleviridae, and Ackermannviridae [8]. In this study, we isolated and characterized two previously undescribed Staphylococcus aureus phages, KSAP7 and KSAP11, belonging to the genus Silviavirus, subfamily Twortvirinae, family Herelleviridae, who
Data Loading...
