Chemical Biology Methods and Protocols
This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemica
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Jonathan E. Hempel Charles H. Williams Charles C. Hong Editors
Chemical Biology Methods and Protocols
METHODS
IN
M O L E C U L A R B I O LO G Y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Chemical Biology Methods and Protocols
Edited by
Jonathan E. Hempel Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN, USA
Charles H. Williams Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN, USA
Charles C. Hong Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN, USA
Editors Jonathan E. Hempel Department of Medicine - Cardiovascular Medicine Vanderbilt Institute of Chemical Biology Vanderbilt University School of Medicine Nashville, TN, USA
Charles H. Williams Department of Cell and Developmental Biology Vanderbilt Institute of Chemical Biology Vanderbilt University School of Medicine Nashville, TN, USA
Charles C. Hong Department of Medicine - Cardiovascular Medicine Department of Pharmacology Department of Cell and Developmental Biology Vanderbilt Institute of Chemical Biology Vanderbilt University School of Medicine Nashville, TN, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-2268-0 ISBN 978-1-4939-2269-7 (eBook) DOI 10.1007/978-1-4939-2269-7 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014959096 © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in t
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