Plant Chemical Genomics Methods and Protocols

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots,

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Friedrich Fauser Martin Jonikas Editors

Plant Chemical Genomics Methods and Protocols

Methods

in

M o l e c u l a r B i o lo g y

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Plant Chemical Genomics Methods and Protocols

Edited by

Friedrich Fauser and Martin Jonikas Department of Molecular Biology, Princeton University, Princeton, NJ, USA

Editors Friedrich Fauser Department of Molecular Biology Princeton University Princeton, NJ, USA

Martin Jonikas Department of Molecular Biology Princeton University Princeton, NJ, USA

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7873-1    ISBN 978-1-4939-7874-8 (eBook) https://doi.org/10.1007/978-1-4939-7874-8 Library of Congress Control Number: 2018940867 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface A grand challenge in plant biology is to understand the functions of genes. The functions of genes can be understood either by introducing stable alterations into the genome or by perturbing cellular processes with small molecules using a chemical biology approach. The past decade has seen the development of a broad variety of high-throughput and “-omics” tools that have accelerated the transition from classical plant chemical genetic strategies into the era of plant chemical genomics. This volume of Methods in Molecular Biology assembles easy-to-follow protocols by experts in the field of plant chemical genomics. We ha