Reverse Chemical Genetics Methods and Protocols
Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Reverse Chemical Genetics Methods and Protocols
Edited by
Hisashi Koga Laboratory of Medical Genomics, Department of Human Genome Research, Kazusa DNA Research Institute, Chiba, Japan
Editor Hisashi Koga Laboratory of Medical Genomics Department of Human Genome Research Kazusa DNA Research Institute Chiba Japan
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-231-5 e-ISBN 978-1-60761-232-2 DOI 10.1007/978-1-60761-232-2 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009933592 Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: Adapted from Figure 5 in Chapter 14 Cover design: Karen Schulz Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface Reverse chemical genetics, in which specific small molecules are used for the analysis, has become a major approach to identifying the functions of certain gene products. It was brought about not only by the discovery of small molecules but also by the advancement of fully characterized genomic resources, such as genome sequencing, cDNA library generation, and siRNA libraries. The availability of complete in silico information through databases is also accelerating research in this field. Reverse chemical genetics corresponds to reverse genetics against forward genetics. In forward chemical genetics, phenotypic changes by molecules precede the identification of genes targeted by specific small molecules. Conversely, in reverse chemical genetics, the identification of target genes precedes phenotypic changes by specific small molecules. We allocated several chapters for the introduction of these fundamental resources, including databases for reverse che
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