Chromatin Protocols
Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, te
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Srikumar P. Chellappan Editor
Chromatin Protocols Third Edition
METHODS
IN
M O L E C U L A R B I O LO G Y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Chromatin Protocols Third Edition
Edited by
Srikumar P. Chellappan H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA
Editor Srikumar P. Chellappan H. Lee Moffitt Cancer Center & Research Institute Tampa, FL, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2473-8 ISBN 978-1-4939-2474-5 (eBook) DOI 10.1007/978-1-4939-2474-5 Library of Congress Control Number: 2015934586 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Primary rat cortical neurons undergoing apoptosis stained with P-Ser10 Histone H3 and MAP2 antibodies; nuclei were visualized using Hoechst staining. Protocol described in Chapter 13. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Spectacular advancements have been made in our knowledge of chromatin structure and function in recent years. The recent development of several novel technologies to interrogate various biological processes has impacted the field of chromatin biology in a remarkable fashion. Thus, the ability to sequence large stretches of DNA, the enhanced capabilities to study protein-DNA interactions at very high resolutions, novel techniques to study histone modifications and other epigenetic changes have all shed new light on our understanding of chromatin structure as well as processes like transcriptional regulation, DNA replication and repair. In addition, the ability to edit genes using CRISPR/CAS technology has opened up new avenues and meth
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