Cigarette smoke extract stimulates bronchial epithelial cells to undergo a SUMOylation turnover
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RESEARCH ARTICLE
Open Access
Cigarette smoke extract stimulates bronchial epithelial cells to undergo a SUMOylation turnover Haifeng Zhou1†, Lei Zhang1,2†, Yang Li1, Guorao Wu1,2, He Zhu1, Huilan Zhang1,2, Jia-Kun Su3, Lei Guo3, Qing Zhou1, Fei Xiong1, Qilin Yu1, Ping Yang1, Shu Zhang1, Jibao Cai3* and Cong-Yi Wang1,2*
Abstract Background: Chronic obstructive pulmonary disease (COPD) characterized by the airway and lung inflammation, is a leading cause of morbidity and mortality worldwide, especially among smokers over 40 years of age and individuals exposed to biomass smoke. Although the detailed mechanisms of this disease remain elusive, there is feasible evidence that protein posttranslational modifications (PTMs) may play a role in its pathoetiology. We thus conducted studies to dissect the effect of cigarette smoke extracts (CSE) on the change of SUMOylated substrates in human bronchial epithelial cells (HBEs). Methods: Samples were collected in HBEs with or without 24 h of CSE insult and then subjected to Western-blot and LC-MS/MS analysis. Subsequently, bioinformatic tools were used to analyze the data. The effect of SUMOylation on cytochrome P450 1A1 (CYP1A1) was evaluated by flow cytometry. Results: It was noted that CSE stimulated HBEs to undergo a SUMOylation turnover as evidenced by the changes of SUMOylated substrates and SUMOylation levels for a particular substrate. The SUMOylated proteins are relevant to the regulation of biological processes, molecular function and cellular components. Particularly, CSE stimulated a significant increase of SUMOylated CYP1A1, a critical enzyme involved in the induction of oxidative stress. Conclusions: Our data provide a protein SUMOylation profile for better understanding of the mechanisms underlying COPD and support that smoking induces oxidative stress in HBEs, which may predispose to the development of COPD in clinical settings. Keywords: Chronic obstructive pulmonary disease, Posttranslational modification, SUMOylation, Cigarette smoke extract, Oxidative stress
* Correspondence: [email protected]; [email protected] † Haifeng Zhou and Lei Zhang contributed equally to this work. 1 The Center for Biomedical Research, Tongji Hospital Research Building, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Caidian 431000, China 3 The Technology Center, China Tobacco Jiangxi Industrial Co., Ltd., Nanchang High Technology Development Valley, Nanchang 330096, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence,
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