Cine interleaved sequences enabled imaging of mice on clinical 3T MRI and analysis of their cardiac function after myoca
- PDF / 215,579 Bytes
- 2 Pages / 595.28 x 793.7 pts Page_size
- 82 Downloads / 166 Views
POSTER PRESENTATION
Open Access
Cine interleaved sequences enabled imaging of mice on clinical 3T MRI and analysis of their cardiac function after myocardial infarction Alexandre Belin1,2*, Vincent Braunersreuther1,2, Fabrizio Montecucco1,2, Benedicte M Delattre1,2, Francois Mach1,2, Jean Vallee1,2 From 15th Annual SCMR Scientific Sessions Orlando, FL, USA. 2-5 February 2012 Background With the poor availability of small animal dedicated MRI, it is of great interest and challenge to use clinical MRI to image and analyze the cardiac function of mice. This would of course be advantageous for translational research and also enable the use of up-to-date sequences already implemented in clinical routine. The aim is to study the time evolution of cardiac function in mice with a myocardial infarct on a clinical MRI. Methods C57BL/6 mice (n=4) were submitted in vivo to left coronary artery permanent ligature and compared and with compared with non-operated mice (n=4). The mice were imaged on a clinical Siemens 3T MRI using an “interleaved” sequence constructed from an ECG-triggered turboflash cine sequence, combining two acquisitions shifted in time yielding an effective time resolution of 6.8 ms and 20-26 phases per heart beat with following parameters: FOV 111 mm, in-plane resolution 257 μm, slice thickness 1 mm, TE/TR 6.2/13.5 ms, flip angle 30°. A soft-thresholding of the temporal Fourier coefficients was used to further denoise the images. The mice were scanned at 24h and 22 days after coronary ligation. Results High quality images suitable for endocardial contouring were obtained for all the animals. The systolic and diastolic volumes, as well as the ejection fraction, are reported in Table 1. The anterior wall of all the operated animals was akenetic as shown on Figure 1. The 1 Radiology, HUG, Geneve 14, Switzerland Full list of author information is available at the end of the article
ejection fraction was significantly decreased in the infarcted group at 24h and 22 days by comparison to the control group (p=0.006 and p=0.003). There was also a trend for the global function to decrease from 24h to 22 days.
Table 1 Cardiac properties of the mice ESV (ul)
EDV (ul)
EF
EVAMR135
65.26
88.12
0.26
EVAMR90
51.27
69.73
0.26
EVAMR174 EVAMR175
43.66 51.27
70.13 81.90
0.38 0.37
MEAN
52.87
77.47
0.32
SD
9.01
9.07
0.07
INF 24h
INF 22j EVAMR135
289.57
356.57
0.19
EVAMR90
208.26
241.62
0.14
EVAMR174 EVAMR175
61.75 66.65
91.41 109.09
0.32 0.39
MEAN
156.56
199.67
0.26
SD
111.71
124.23
0.12
P1
21.13
56.86
0.63
P2
33.92
69.12
0.51
P3
36.83
79.46
0.54
P4 MEAN
47.59 34.87
90.64 74.02
0.47 0.54
SD
10.88
14.43
0.07
NON INF
© 2012 Belin et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Belin et al. Journal of Cardio
Data Loading...
