Clinical significance of serum miR-768-3p in HBV-related hepatocellular carcinoma and its potential mechanism
- PDF / 908,494 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 41 Downloads / 149 Views
ORIGINAL ARTICLE
Clinical significance of serum miR‑768‑3p in HBV‑related hepatocellular carcinoma and its potential mechanism Chunyu Cao1 · Changjiang Wang2 Received: 18 May 2020 / Accepted: 13 July 2020 © Springer Nature Switzerland AG 2020
Abstract The purpose of this study was to reveal the clinical diagnostic and prognostic value of miR-768-3p in HBV-related HCC and to investigate its effect on the biological function of HCC. Quantitative real-time polymerase chain reaction was used to detect the expression level of miR-768-3p in subjects’ serum. The receiver operating characteristics curve (ROC) evaluated the diagnostic value of miR-768-3p in patients. A Chi-square test was used to analyze the relationship between miR-768-3p and clinical data of patients. Kaplan–Meier survival and Cox regression analysis assessed the prognostic value of miR768-3p in HCC. Finally, CCK-8 and Transwell assays were used to demonstrate the effect of miR-768-3p on HBV-related HCC function. Serum miR-768-3p was significantly lower in HCC patients than in healthy controls and chronic hepatitis B (CHB) patients. ROC curve suggested that serum miR-768-3p has an important diagnostic value for HBV-related HCC and can significantly differentiate HCC patients from healthy controls, and it can also diagnose HCC patients from CHB patients. Cox analysis confirmed that miR-768-3p was an independent risk factor. Low expression of miR-768-3p was associated with Tumor, Node, Metastasis stage, Barcelona Clinic Liver Cancer stage, and poor prognosis in HCC patients. Finally, cell function experiments confirmed that high expression of miR-768-3p could inhibit cell proliferation, migration, and invasion. All experiments confirmed that miR-768-3p can inhibit the proliferation, migration, and invasion of HBV-related HCC cells, and the low expression of miR-768-3p can be used as a potential diagnostic and prognostic biomarker for HBV-related HCC. Keywords miR-768-3p · Hepatocellular carcinoma · Hepatitis B virus · Diagnostic · Prognostic Abbreviations HCC Hepatocellular carcinoma HBV Hepatitis B virus RFA Radiofrequency ablation AFP Alpha-fetoprotein miRNA MicroRNA CHB Chronic hepatitis B BCLC Barcelona Clinic Liver Cancer FBS Fetal bovine serum qRT-PCR Real-time fluorescence quantitative PCR SD Standard deviation ROC Receiver operating characteristic
* Changjiang Wang [email protected] 1
Department of Infectious Diseases, Caoxian People’s Hospital, Heze 274400, Shandong, China
Department of Gastroenterology, Caoxian People’s Hospital, No. 1, Fuminda Road, Heze 274400, Shandong Province, China
2
Introduction Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide, the fifth and seventh most common cancers in men and women, and more than 700,000 patients die from the disease each year worldwide [1, 2]. Hepatitis B virus (HBV) infection is an important risk factor for this cancer and is associated with an 80% incidence of HCC [3]. China, as one of the more prevalent countries of HBV, acc
Data Loading...
