Clinical status determines the efficacy of salivary and nasopharyngeal samples for detection of SARS-CoV-2
- PDF / 163,760 Bytes
- 2 Pages / 595.276 x 790.866 pts Page_size
- 63 Downloads / 191 Views
LETTER TO THE EDITOR
Clinical status determines the efficacy of salivary and nasopharyngeal samples for detection of SARS-CoV-2 Gargi S. Sarode 1 & Sachin C. Sarode 1 & Namrata Sengupta 1 & Amol R. Gadbail 2 & Shailesh Gondivkar 3 & Nilesh Kumar Sharma 4 & Shankargouda Patil 5 Received: 26 September 2020 / Accepted: 7 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Recently, salivary samples have been widely investigated for the detection of SARS-CoV-2 RNA with variable success rate [1–9]. The rationale claims that the virus particles possibly come from the respiratory system and infected salivary glands [1]. Proposed advantages are less-invasive, convenience, selfcollection, and minimum risk of cross infection [2]. To draw a meaningful conclusion in this regard, the most important study design would be a comparative cross-sectional analysis of salivary and nasopharyngeal samples (NPSs) in the detection of SARS-CoV-2 RNA with a cycle threshold value. Hence, we decided to critically analyze the results of published papers with such a study design. We searched PubMed, SCOPUS, and Web of Science databases with keywords such as COVID-19, SARS-CoV-2, saliva, and nasopharyngeal swab in various permutations and combinations to retrieve the papers exclusively on the comparative analysis of saliva and NPS for detection of SARS-CoV-2 RNA using RT-PCR. After a thorough literature search, we could able to shortlist a total of nine studies from the literature [1–9]. Data such as sample size, disease * Sachin C. Sarode [email protected] 1
Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune, MH 411018, India
2
Department of Dentistry, Indira Gandhi Government Medical College and Hospital, Nagpur, Maharashtra, India
3
Department of Oral Medicine and Radiology, Government Dental College & Hospital, Nagpur, Maharashtra, India
4
Cancer and Translational Research Lab, Dr. D.Y. Patil Biotechnology & Bioinformatics Institute, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra 411033, India
5
Division of Oral Pathology, Department of Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia
status, detection rate, and cycle threshold value were retrieved. (Table 1) All the studies projected saliva as potential sampling material for the detection and diagnosis of SARS-CoV-2 RNA using RT-PCR. One interesting trend apprehended our attention is the detection rate and cycle threshold values in symptomatic and asymptomatic patients. The categorization and characterization of the symptomatic and asymptomatic status of the patient were available in four [1, 4, 5, 9] and two [2, 7] cases, respectively. In asymptomatic cases, the sensitivity and detection rate was more in salivary samples as compared to NPS [2, 7]. Moreover, the cycle threshold values were comparatively low in salivary samples depicting high viral load in the oral cavity, whereas studies on sympto
Data Loading...
