Cloning and expression analysis of hypoxia-related gene HO in cobia
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Cloning and expression analysis of hypoxia-related gene HO in cobia Rui-tao Xie 1 & Eric Amenyogbe 1 & Wei-zheng Wang 1 & Zhi-xiong Guo 1 & Gang Chen 1,2,3 & Jian-sheng Huang 1,2,3 Received: 14 April 2020 / Accepted: 24 September 2020/ # Springer Nature Switzerland AG 2020
Abstract
The heme oxygenase gene plays a role in many biological processes and is an important protective factor in hypoxic stress. Research on the HO gene of fishes has attracted increasing attention, but there is yet little information about the cobia (Rachycentron canadum) heme oxygenase gene. In this study, the full-length cDNA of the cobia heme oxygenase gene (heme oxygenase, HO) was cloned using rapid amplification of cDNA ends technology. The full-length cDNA was 1639 bp and the open reading frame (ORF) was 840 bp encoding 279 amino acids, with 5′-UTR 518 bp and 3′-UTR 281 bp. The results of real-time quantitative PCR showed that cobia HO was expressed in all the tissues tested, with the highest expression in gills. The mRNA expression of gill HO increased after hypoxia stress. There was no significant change in the expression of HO mRNA in the liver after hypoxia stress. The amino acid sequences of HO in diverse fish and mammals have varying levels of homology. Our results indicate that the biological functions of HO may be similar in fish and mammals, suggesting an important protective factor in response to hypoxic stress. Keywords Gene cloning . Heme oxygenase gene . Hypoxia stress . mRNA expression . Rachycentron canadum
Rui-tao Xie and Eric Amenyogbe contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10499-02000611-3) contains supplementary material, which is available to authorized users.
* Jian-sheng Huang [email protected]
1
Fishery College, Guangdong Ocean University, Zhanjiang 524025, China
2
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China
3
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China
Aquaculture International
Introduction In recent decades, human activities and climate change have rendered hypoxia in estuaries and coastal waters more severe. This is reflected in the increased frequency of hypoxia, larger areas of affected ocean, and prolonged periods of low dissolved oxygen. Hypoxia significantly affects the movement, feeding, reproduction, and survival of marine life, altering energy flow through food webs and overall ecosystem function (Xiao 2015; Bowden 2008; Zhu et al. 2013; Chen et al. 2016). Low dissolved oxygen in water has effects on fishes at many biological levels (Wang et al. 2012). For example, hypoxia can significantly affect different cellular and molecular processes in fish. It can cause changes in proteins and cytokines which will affect the physiological, biochemical, and behavioral activities of fish, ultimately cascading to alter the biological characteristics of entire populations (Xia
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