Molecular cloning and characterization of HSP60 gene in domestic pigeons ( Columba livia ) and differential expression p
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ORIGINAL PAPER
Molecular cloning and characterization of HSP60 gene in domestic pigeons (Columba livia) and differential expression patterns under temperature stress Jianke Yang 1,2 & Juan Gu 1,3 & Yuqing Hu 1,4 & Nan Wang 1,4 & Jiguang Gao 1,2 & Ping Wang 1 Received: 20 April 2020 / Revised: 19 August 2020 / Accepted: 25 August 2020 # Cell Stress Society International 2020
Abstract Heat shock protein 60 (HSP60) is a well-recognized multifunctional protein, playing a substantial role in protecting organisms from environmental stress. The domestic pigeon (Columba livia) is a promising model organism, with important economic and ecological value, and its health is susceptible to temperature stress. To explore the molecular characteristics, tissue expression profile, and response to temperature stress for HSP60 of Columba livia (ClHSP60), we firstly cloned and characterized the complete cDNA sequence and investigated its expression profile under optimal conditions and acute temperature stress. The cDNA of ClHSP60 contained 2257 nucleotides, consisting of 12 exons with length ranging from 65 to 590 bp. The open reading frame (ORF) encoded 573 amino acids with calculated molecular weight of 60.97 kDa that contained a number of structurally prominent domains or motifs. Under optimal temperature conditions, levels of ClHSP60 expression differed between all the tested tissues (the highest was noted in liver and the lowest in pectoralis major muscle). Under acute temperature stress, five patterns of change were detected in the tested tissues, suggesting that different tissues in domestic pigeons differentially responded to various temperature stress conditions. Upregulation of ClHSP60 expression was highest in the lung and pectoralis major muscle, reflecting the crucial role of these two tissues in temperature regulation. However, the crop, cerebrum, and heart showed little change or decreased ClHSP60 expression. The results indicate that ClHSP60 may be sensitive to and play pivotal roles in responding to acute temperature stress. Keywords HSP60 . Domestic pigeon . Differential expression . Temperature stress
Introduction Heat shock proteins (HSPs), also known as stress proteins or chaperone proteins, are a group of highly conserved proteins Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12192-020-01160-7) contains supplementary material, which is available to authorized users. * Ping Wang [email protected] 1
School of Preclinical Medicine, Wannan Medical College, Wuhu 241001, China
2
Research laboratory of Tumor Microenvironment, Wannan Medical College, Wuhu 241001, China
3
School of pharmacy, Wannan Medical College, Wuhu 241001, China
4
School of Clinical Medicine, Wannan Medical College, Wuhu 241001, China
from prokaryotes to eukaryotes, which play crucial roles in protein homeostasis, signal transduction, and immune response (Feder and Hofmann 1999; Lindquist 1986). They can also be induced and rapidly synthesized to protect organisms from injury caused by en
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