Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8 + T cell activation in
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Journal of Translational Medicine Open Access
RESEARCH
Closing the system: production of viral antigen‑presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags Jean‑Philippe Bastien2†, Natalie Fekete1†, Ariane V. Beland1, Marie‑Paule Lachambre2, Veronique Laforte3,4,5, David Juncker3,4,5, Vibhuti Dave2,6, Denis‑Claude Roy2,7† and Corinne A. Hoesli1,3*†
Abstract Background: A major obstacle to anti-viral and -tumor cell vaccination and T cell immunotherapy is the ability to produce dendritic cells (DCs) in a suitable clinical setting. It is imperative to develop closed cell culture systems to accelerate the translation of promising DC-based cell therapy products to the clinic. The objective of this study was to investigate whether viral antigen-loaded monocyte-derived DCs (Mo-DCs) capable of eliciting specific T cell activa‑ tion can be manufactured in fluorinated ethylene propylene (FEP) bags. Methods: Mo-DCs were generated through a protocol applying cytokine cocktails combined with lipopolysac‑ charide or with a CMV viral peptide antigen in conventional tissue culture polystyrene (TCPS) or FEP culture ves‑ sels. Research-scale (
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