Co-administration of Selenium Nanoparticles and Metformin Abrogate Testicular Oxidative Injury by Suppressing Redox Imba
- PDF / 3,464,560 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 173 Views
Co-administration of Selenium Nanoparticles and Metformin Abrogate Testicular Oxidative Injury by Suppressing Redox Imbalance, Augmenting Sperm Quality and Nrf2 Protein Expression in Streptozotocin-Induced Diabetic Rats Azubuike P. Ebokaiwe 1 & Kebe E. Obeten 2 & Stephen O. Okori 2 & Ebuka E. David 1 & Olasehinde Olusanya 3 & Chukwuma J. Chukwu 1 & Nworie Okoro 4 & Richard C. Ehiri 1 Received: 22 November 2019 / Accepted: 14 February 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Selenium nanoparticles (SeNPs) and metformin (Met) elicit individually protective effects against testicular oxidative injury in diabetic rats. However, the combined effects of both compounds have not been investigated. We investigated the effects of SeNPs and Met individual/co-treatment on testicular oxidative injury in diabetic rats. Diabetes was induced by a single intraperitoneal administration of streptozotocin (STZ-40 mg/kg bwt). The rats were equally divided into 6 groups: Group one—non-diabetic; group two—diabetic untreated; and group six—non-diabetic received citrate buffer (2 mL/kg bwt), while group three, four, and five received SeNPs (0.1 mg/kg bwt), Met (50 mg/kg bwt), and SeNPs/Met combined respectively, for 42 days. Results revealed that SeNPs, as well as Met treatment significantly (p < 0.001), lowered blood glucose levels and improved relative organ weights in treated rats than those of the untreated group. Moreover, a synergistic effect was observed in the co-administration group. Additionally, combined treatment elicited better effect, in augmenting the pituitary and testicular hormone (LH, FSH, prolactin, and testosterone) levels, marker enzymes/protein associated with steroidogenesis (3-βHSD, 17-βHSD, and StAR protein), and sperm functional parameters than those of individual treatment groups, when compared with control. Furthermore, the combinatorial effects of SeNPs and Met surpassed their influence in attenuating testicular oxidative stress/inflammation and upregulation of Nrf2 protein expression in diabetic rats when compared with control. Overall, normal rats, co-treated with SeNPs and Met, did not reveal any deleterious effect. Therefore, SeNPs and Met combined treatment may better improve testes function in diabetic conditions than an individual regimen. Keywords Diabetes . Testes . Selenium nanoparticles . Metformin
Introduction * Azubuike P. Ebokaiwe [email protected]; [email protected] 1
Department of Chemistry/Biochemistry and Molecular Biology, Alex Ekwueme Federal University, Ndufu-Alike Ikwo, PMB 1010, Abakaliki, Ebonyi State, Nigeria
2
Department of Anatomy, Cross River University of Technology, Cross River State, Okuku, Nigeria
3
Department of Biochemistry, University of Benein, Benin City, Nigeria
4
Department of Biology/Microbiology/Biotechnology, Alex-Ekwueme Federal University, Ndufu-Alike, Nigeria
The twin process of spermatogenesis and steroidogenesis that characterize male reproductive function is a targeted physiological process, often imp
Data Loading...
