Zygophyllum album leaves extract prevented hepatic fibrosis in rats, by reducing liver injury and suppressing oxidative
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Inflammopharmacology
ORIGINAL ARTICLE
Zygophyllum album leaves extract prevented hepatic fibrosis in rats, by reducing liver injury and suppressing oxidative stress, inflammation, apoptosis and the TGF‑β1/Smads signaling pathways. Exploring of bioactive compounds using HPLC–DAD–ESI–QTOF‑MS/MS Anouar Feriani1 · Meriam Tir2 · Ana María Gómez‑Caravaca3 · María del Mar Contreras3,4 · Amani Taamalli5 · Antonio Segura‑Carretero3 · Lakhdar Ghazouani1 · Afoua Mufti1 · Nizar Tlili6,7 · Abdelfattah El Feki8 · Abdel Halim Harrath9 · Mohamed Salah Allagui8 Received: 9 December 2019 / Accepted: 10 March 2020 © Springer Nature Switzerland AG 2020
Abstract Zygophyllum album is traditionally used against many illnesses, such as liver disease. The present study investigated the bioactive compounds in methanol extract of Z. album (MEZA) using HPLC–DAD–ESI–QTOF-MS/MS and explored its possible antioxidative, anti-inflammatory, anti-apoptotic, and hepatoprotective effect. Twelve phenolic compounds were identified; isorhamnetin-3-O-rutinoside being the main one was the main composite (144.6 mg/100 g dm). Results showed that MEZA reduced significantly the biochemical markers (AST, ALT, LDH and ALP), and the hepatic oxidative stress indicators (MDA, PC, SOD, CAT, and GPx) in deltamethrin (DLM)-treated rats. Moreover, MEZA limited the inflammatory responses through downregulation of NF-κB gene, which suppressed the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6). Furthermore, Z. album reduced DLM-induced apoptosis by attenuating caspase 3 and p53 mRNA activation. MEZA treatment also alleviated upregulation of α-SMA, type I collagen, and TGF-β1 mRNA in the liver. The possible antifibrotic effect of MEZA was clearly demonstrated by the histopathology examination, using Masson’s Trichrome and Sirius Red stainings. Therefore, the current study suggested that the bioactive compounds of Z. album possessed antifibrotic effect against DLM-induced hepatic fibrosis, by protecting liver tissue, and inhibiting oxidative stress, inflammation, apoptosis and the TGF-β1/Smads signaling pathways. Keywords Zygophyllum album · Inflammation · Oxidative stress · Fibrosis · Apoptosis · HPLC–DAD–ESI–QTOF-MS
* Meriam Tir [email protected] 1
Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia
Laboratoire D’Ecologie, de Biologie Et de Physiologie Des Organismes Aquatiques, LR18ES41, Faculté Des Sciences de Tunis, Université Tunis EL Manar, 2092 Tunis, Tunisia
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Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva S/N, 18071 Granada, Spain Department of Chemical, Environmental and Material Engineering, University of Jaén, Campus Las Lagunillas, s/n, 23071 Jaén, Spain
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Laboratory of Olive Biotechnology, Center of Biotechnology of Borj-Cédria, BP. 901, 2050 Hammam‑Lif, Tunisia
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Institut Supérieur des Sciences et Technologies de l’Environnement, ISSTE de Borj Cédria, Université de Carthage, 2050 Hammam‑Lif, Tunisia
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Faculté Des Sci
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